Recombinant Human NG2/MCSP/CSPG4 Protein, CF Summary
Product Specifications
Ser1583-Ser2224, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2585-PG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 300 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: NG2/MCSP
NG2, also known as CSPG4, MCSP, and AN2, is a 400-500 kDa transmembrane chondroitin sulfate proteoglycan (CSPG) with a protein core of approximately 300 kDa. The extracellular region can be proteolytically shed from the cell surface (1-3). Mature human NG2 consists of a 2195 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (4). Within aa 1583-2224, human NG2/CSPG4 shares 83% aa sequence identity with mouse and rat CSPG4. NG2 binds to the extracellular matrix proteins Laminin, Tenascin, and Collagens II, V, and VI as well as to the growth factors FGF-2 and PDGF-AA (1, 5, 6). NG2 is expressed on glial cell progenitors known as O2A cells or NG2 glia (2, 7, 8). These cells are neuronally responsive and differentiate primarily into oligodendrocytes but also into astrocytes (8-10). NG2 associates with PDGF R alpha and the AMPA R subunit GluR2 (2, 7). It is up-regulated on microglial cells during inflammation and contributes to the induction of inflammatory mediators (11). Various CSPGs in the brain inhibit neurite outgrowth through interactions with Nogo Receptor/NgR1 and NgR3 (12). This recombinant protein product corresponds to the last 5 CSPG repeats, a region which can independently inhibit neurite outgrowth (13). NG2 is also expressed on vascular mural cells and capillaries (10, 14). It promotes vascular endothelial cell (EC) migration and angiogenesis through interactions with Galectin-3 and Integrin alpha 3 beta 1 on EC, Plasminogen, and Angiostatin (15, 16). NG2 is also expressed on a variety of tumors where it contributes to tumor cell adhesion, motility, and invasion (17).
- Campoli, M. et al. (2010) Adv. Cancer Res. 109:73.
- Nishiyama, A. et al. (1996) J. Neurosci. Res. 43:315.
- Nishiyama, A. et al. (1995) Mol. Biol. Cell 6:1819.
- Pluschke, G. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9710.
- Burg, M.A. et al. (1996) J. Biol. Chem. 271:26110
- Goretzki, L. et al. (1999) J. Biol. Chem. 274:16831.
- Stegmuller, J. et al. (2003) J. Biol. Chem. 278:3590.
- Komitova, M. et al. (2009) J. Comp. Neurol. 512:702.
- Bergles, D.E. et al. (2000) Nature 405:187.
- Zhu, X. et al. (2008) Development 135:145.
- Gao, Q. et al. (2010) Neuroscience 165:386.
- Dickendesher, T.L. et al. (2012) Nat. Neurosci. 15:703.
- Ughrin, Y.M. et al. (2003) J. Neurosci. 23:175.
- Schlingemann, R.O. et al. (1990) Am. J. Pathol. 136:1393.
- Fukushi, J. et al. (2004) Mol. Biol. Cell 15:3580.
- Goretzki, L. et al. (2000) J. Biol. Chem. 275:28625.
- Wang, X. et al. (2010) Curr. Mol. Med. 10:419.
Citations for Recombinant Human NG2/MCSP/CSPG4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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iPS-Derived Early Oligodendrocyte Progenitor Cells from SPMS Patients Reveal Deficient In Vitro Cell Migration Stimulation
Authors: L Lopez-Cara, J Martorell-, P Carmona-Sá, E Gonzalez-M
Cells, 2020-07-29;9(8):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A novel excision selection method for isolation of antibodies binding antigens expressed specifically by rare cells in tissue sections
Authors: S Lykkemark, OA Mandrup, MB Jensen, J Just, P Kristensen
Nucleic Acids Res., 2017-06-20;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard)
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