Recombinant Human NPDC-1 Protein, CF Summary
Product Specifications
Gly35-Asp181, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9724-NP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: NPDC-1
Neural proliferation and differentiation control protein 1 (NPDC1) is an approximately 34 kDa protein that is mainly expressed in lung and neural tissues and whose expression is developmentally regulated and persists in the adult (1, 2). It binds a variety of cyclins and regulate differentiation events in neuronal precursor cells (3). NPDC1 also binds the transcription factor, E2F-1, which results in the inhibition of E2F-1 binding to DNA and the corresponding transcription events (4). The encoded protein of the human NPDC1 includes a 34 aa signal peptide, a 147 aa extracellular domain (ECD), a 21 aa transmembrane region, followed by a carboxyl terminus containing a PEST motif (rich in proline, glutamine, serine, and threonine) which targets the protein for degradation (1). Alignment of the mature protein shows that human NPDC1 shares 76% and 75% sequence identity with mouse and rat NPDC1, respectively.
- Spencer, M.L. et al. (2004) J. Biol. Chem. 279:37069.
- Evrard, C. and P. Rouget (2005) J. Neurosci. Res. 79:747.
- Galiana, E. et al. (1995) Proc. Natl. Acad. Sci. USA. 92:1560.
- Sansal, I. et al. (2000) Oncogene 19:5000.
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