Recombinant Human Syndecan-1 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI10913 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10913-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Syndecan-1 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Human Syndecan-1/CD138 Antibody (Catalog # MAB2780) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human Syndecan-1/CD138 Fc Chimera Avi-tag (Catalog# AVI10913 binds with an ED₅₀ of 0.3-2.1 ng/mL. Measured by the ability of the immobilized protein to support the adhesion of Saos-2 human osteosarcoma cells. The ED₅₀for this effect is 0.5-6.0 µg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Syndecan-1/CD138 protein

Human Syndecan-1 (Gln18-Gln251) Accession # AAH08765.1	IEGRMD	Human IgG1 Fc (Pro 100-Lys 330)	Avi-tag
N-terminus			C-terminus

Accession #

AAH08765.1

N-terminal Sequence
Analysis

Gln 18, determined by protein ID

Structure / Form

Biotinylated via Avi-tag.

Predicted Molecular Mass

53 kDa

SDS-PAGE

81-105 kDa, under reducing conditions.

Product Datasheets

AVI10913

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10913

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Human Syndecan-1/CD138 Antibody (MAB2780) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human Syndecan-1/CD138 Fc Chimera Avi-tag (Catalog # AVI10913) binds with an ED₅₀of 0.3-2.1 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human Syndecan‑1/CD138 Fc Chimera Avi-tag Protein (Catalog # AVI10913) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 81-105 kDa and 160-210 kDa, respectively..

Reconstitution Calculator

Background: Syndecan-1/CD138

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the syndecan family of Type 1 transmembrane proteins (1, 2). The four syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 17 aa signal sequence, a 234 aa extracellular domain (ECD) that includes three closely-spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 25 aa TM segment, and a 34 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site. The ECD is variably modified by GAGs, producing molecular weights of 120 - 200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Human Syndecan-1 ECD shares 65 - 71% aa identity with the ECD of rat, mouse, canine, equine and bovine Syndecan-1. Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (4, 5). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (4 - 8). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (5, 7, 9). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (10 - 12). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (12, 13). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (4). Our Avi-tag Biotinylated human Syndecan-1/CD138 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Tkachenko, E. et al. (2005) Circ. Res. 96:488.
Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
Vanhoutte, D. et al. (2007) Circulation 115:475.
Li, Q. et al. (2002) Cell 111:635.
Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
Derksen, P.W.B. et al. (2002) Blood 99:1405.
Su, G. et al. (2007) J. Biol. Chem. 282:14906.

Entrez Gene IDs

6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)

Alternate Names

CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1