Recombinant Human Tau 383 (0N4R) Protein, CF
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Recombinant Human Tau 383 (0N4R) Protein, CF Summary
Product Specifications
Met1 - Leu383
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
SP-499
| Formulation | Supplied as a solution in PBS. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Tau
Tau is a microtubule-associated protein expressed primarily in neurons. Carboxy-terminal domains of Tau associate with and stabilize microtubule structure, while other domains bind to the plasma membrane. Abnormal Tau phosphorylation may result in the self-assembly of tangles of paired helical and/or straight filaments, which are involved in the pathogenesis of Alzheimer's disease and other neurodegenerative diseases. Properly folded Tau is highly soluble, but when the protein becomes misfolded it forms insoluble aggregates that can damage cytoplasmic functions, interfere with axonal transport and ultimately lead to cell death. There are multiple forms of Tau. The product is a 383 amino acid isoform known as "0N4R," "Isoform Tau-D" or "Tau 383" and is referenced in UniProt as P10636-6.
- Billingsley M.L. & Kincaid R.L. (1997) Biochem. J. 323:577.
- Bloom G.S. (2014) JAMA Neurol. 71:505.
- Cripps D. et al. (2006) J. Biol. Chem. 281:10825.
- Harada A. et al. (1994) Nature 369:488.
- Lei P. et al. (2010) Int. J. Biochem. Cell Biol. 42:775.
Citation for Recombinant Human Tau 383 (0N4R) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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The autophagy adaptor TRIAD3A promotes tau fibrillation by nested phase separation
Authors: Zhou, J;Chuang, Y';Redding-Ochoa, J;Zhang, R;Platero, AJ;Barrett, AH;Troncoso, JC;Worley, PF;Zhang, W;
Nature cell biology
Species: N/A
Sample Types: Recombinant Protein
Applications: Western Blot
FAQs
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Does Recombinant Human Tau 383 (0N4R) Protein, CF (Catalog # SP-499) contain a Caspase-3 cleaved form?
The Tau protein can get cleaved by the enzyme Caspase-3 after the amino acid residues DEVD or DMVD. The Tau 383 isoform does contain that sequence of residues ending at aa363. However, SP-499 is raised in E. coli, which does not naturally express Caspase-3. Therefore, we would not expect the cleaved form of Tau 383 to be present in SP-499.
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