Recombinant Human Tenascin C Protein, CF Summary
Product Specifications
Gly23-Pro625, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3358-TC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Tenascin C
Tenascin C, also known as hexabrachion, cytotactin, neuronectin, GMEM, JI, myotendinous antigen, glioma-associated-extracellular matrix antigen, and GP 150-225, is a member of the Tenascin family of extracellular matrix proteins. It is secreted as a disulfide-linked homohexamer whose subunits can vary in size from approximately 200 kDa to over 300 kDa due to differences in glycosylation (1). Rotary-shadowed electron micrographs of the purified molecule show six strands joined to one another at one end in a globular domain with each arm terminating in a knob-like structure (2-3). The human Tenascin C monomer is synthesized as a precursor with a 22 amino acid (aa) signal sequence and a 2179 aa mature chain (SwissProt # P24821). The mature chain consists of a coiled-coil region (aa 118-145), followed by 15 EGF-like domains, 15 fibronectin type-III domains, and a fibrinogen C-terminal domain. In addition, there are 23 potential sites of N-linked glycosylation. Alternative splicing within the fibronectin type-III repeats produces six isoforms for human Tenascin C. Mature human Tenascin C (isoform 1) shares 84% aa sequence identity with mature mouse Tenascin C. In the developing embryo, Tenascin C is expressed during neural, skeletal, and vascular morphogenesis (1, 2). In the adult, it virtually disappears with continued basal expression detectable only in tendon-associated tissues (1, 2). However, greatup-regulation in expression occurs in tissues undergoing remodeling processes seen during wound repair and neovascularization or in pathological states such as inflammation or tumorigenesis (1, 4-5). Biologically, Tenascin C functions as an adhesion-modulatory extracellular matrix protein (1, 4-8). Specifically, it antagonizes the adhesive effects of fibronectin, and impacts the ability of fibroblasts to deposit and contract the matrix by affecting the morphology and signaling pathways of adherent cells (5-7). Tenascin C acts by blocking syndecan-4 binding at the edges of the wound and by suppressing fibronectin-mediated activation of RhoA and focal adhesion kinase (FAK) (4-8). Tenascin C thus promotes epidermal cell migration and proliferation during wound repair.
- Hsia, H.C. and J.E. Schwarzbauer (2005) J. Biol. Chem. 280:26641.
- Nies, D.E. et al. (1991) J. Biol. Chem. 266:2818.
- Erickson, H.P and J.L. Iglesias (1984) Nature 311:267.
- Orend, G. et al. (2003) Oncogene 22:3917.
- Wenk, M.B. et al. (2000) J. Cell Biol. 150:913.
- Midwood, K.S. et al. (2004) Mol. Biol. Cell 15:5670.
- Midwood, K.S. and J. E. Schwarzbauer (2002) Mol. Biol. Cell 13:3601.
- Hsia, H.C. and J.E. Schwarzbauer (2006) J. Surg. Res. 136:92.
Citations for Recombinant Human Tenascin C Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Harnessing developmental dynamics of spinal cord extracellular matrix improves regenerative potential of spinal cord organoids
Authors: Sun, Z;Chen, Z;Yin, M;Wu, X;Guo, B;Cheng, X;Quan, R;Sun, Y;Zhang, Q;Fan, Y;Jin, C;Yin, Y;Hou, X;Liu, W;Shu, M;Xue, X;Shi, Y;Chen, B;Xiao, Z;Dai, J;Zhao, Y;
Cell stem cell
Species: Rabbit, Rat
Sample Types: Organoid
Applications: Bioassay -
Tenascin C+ papillary fibroblasts facilitate neuro-immune interaction in a mouse model of psoriasis
Authors: X Cai, M Han, F Lou, Y Sun, Q Yin, L Sun, Z Wang, X Li, H Zhou, Z Xu, H Wang, S Deng, X Zheng, T Zhang, Q Li, B Zhou, H Wang
Nature Communications, 2023-04-10;14(1):2004.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Intrahepatic Cholangiocarcinoma Developing in Patients with Metabolic Syndrome Is Characterized by Osteopontin Overexpression in the Tumor Stroma
Authors: M Cadamuro, S Sarcognato, R Camerotto, N Girardi, A Lasagni, G Zanus, U Cillo, E Gringeri, G Morana, M Strazzabos, E Campello, P Simioni, M Guido, L Fabris
International Journal of Molecular Sciences, 2023-03-01;24(5):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Identification of anti-citrullinated osteopontin antibodies and increased inflammatory response by enhancement of osteopontin binding to fibroblast-like synoviocytes in rheumatoid arthritis
Authors: A Umemoto, T Kuwada, K Murata, M Shiokawa, S Ota, Y Murotani, A Itamoto, K Nishitani, H Yoshitomi, T Fujii, A Onishi, H Onizawa, K Murakami, M Tanaka, H Ito, H Seno, A Morinobu, S Matsuda
Arthritis Research & Therapy, 2023-02-17;25(1):25.
Species: Human, N/A
Sample Types: Antibody, Protein
Applications: Bioassay, ELISA Capture -
Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids
Authors: S Kim, S Min, YS Choi, SH Jo, JH Jung, K Han, J Kim, S An, YW Ji, YG Kim, SW Cho
Nature Communications, 2022-03-30;13(1):1692.
Species: Human
Sample Types: Extracellular Matrix
Applications: Bioassay -
mTOR inhibition prevents angiotensin II-induced aortic rupture and pseudoaneurysm but promotes dissection in Apoe-deficient mice
Authors: C He, B Jiang, M Wang, P Ren, SI Murtada, AW Caulk, G Li, L Qin, R Assi, CJ Lovoulos, MA Schwartz, JD Humphrey, G Tellides
JCI Insight, 2022-02-08;7(3):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration
Authors: M Takeo, K Asakawa, KE Toyoshima, M Ogawa, J Tong, T Irié, M Yanagisawa, A Sato, T Tsuji
Scientific Reports, 2021-02-10;11(1):1173.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Microenvironmental Factors Drive Tenascin C and Src Cooperation to Promote Invadopodia Formation in Ewing Sarcoma
Authors: AG Hawkins, CM Julian, S Konzen, S Treichel, ER Lawlor, KM Bailey
Neoplasia, 2019-09-13;21(10):1063-1072.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Acetyl-CoA promotes glioblastoma cell adhesion and migration through Ca2+-NFAT signaling
Authors: JV Lee, CT Berry, K Kim, P Sen, T Kim, A Carrer, S Trefely, S Zhao, S Fernandez, LE Barney, AD Schwartz, SR Peyton, NW Snyder, SL Berger, BD Freedman, KE Wellen
Genes Dev., 2018-04-19;32(7):497-511.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Targeted disruption of the iNOS gene improves adipose tissue inflammation and fibrosis in leptin-deficient ob/ob mice: role of tenascin C
Authors: S Becerril, A Rodríguez, V Catalán, L Méndez-Gim, B Ramírez, N Sáinz, M Llorente, X Unamuno, J Gómez-Ambr, G Frühbeck
Int J Obes (Lond), 2018-02-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay, Cell Culture -
Activation of NOTCH signaling by tenascin-C promotes growth of human brain tumor-initiating cells
Authors: S Sarkar, R Mirzaei, FJ Zemp, W Wu, DL Senger, SM Robbins, VW Yong
Cancer Res., 2017-04-17;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Integrins functioning in uterine endometrial stromal and epithelial cells in estrus
Authors: Hye Jin Park
Reproduction, 2016-12-20;0(0):.
Applications: Bioassay -
Migration of breast cancer cell lines in response to pulmonary laminin 332
Cancer Med, 2016-11-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
J Immunol, 2015-01-02;194(3):1039-46.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.
Authors: Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y
Blood, 2012-05-02;119(23):5429-37.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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Reason for Rating: It works well for growing cells.
