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Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Catalog #: 10291-TA
8 Citations 4 Reviews Datasheet / COA / SDS
Analyzed by SEC-MALS
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10291-TA-020
10291-TA-050
10291-TA-100
10291-TA-250
10291-TA-500
10291-TA-01M
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Recombinant Human TNF-alpha (HEK293-expressed) Protein SEC-MALS
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Product Details
Citations (8)
FAQs
Reviews (4)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 20-100 pg/mL.
Source
Human embryonic kidney cell, HEK293-derived human TNF-alpha protein
Val77-Leu233
Accession #
P01375
N-terminal Sequence
Analysis
Val77
Predicted Molecular Mass
17 kDa
SDS-PAGE
14-18 kDa, under reducing conditions

Product Datasheets

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10291-TA

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10291-TA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant human TNF-alpha (Catalog # 10291-TA) has a molecular weight (MW) of 52.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Bioactivity Recombinant Human TNF-alpha (HEK293-expressed) Protein Bioactivity View Larger

Recombinant Human TNF‑ alpha (HEK293-expressed) (Catalog # 10291-TA) induces cytotoxicity in the L‑929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 20‑100 pg/mL.

SDS-PAGE Recombinant Human TNF-alpha (HEK293-expressed) Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human TNF-alpha (HEK293-expressed) (Catalog # 10291-TA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).

References
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Pennica, D. et al. (1984) Nature 312:724.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.
Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Citations for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. TNF Induces Laminin-332-Encoding Genes in Endothelial Cells and Laminin-332 Promotes an Atherogenic Endothelial Phenotype
    Authors: Hayderi, A;Zegeye, MM;Meydan, S;Sirsjö, A;Kumawat, AK;Ljungberg, LU;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Blood DNA methylation profiling identifies cathepsin Z dysregulation in pulmonary arterial hypertension
    Authors: Ulrich, A;Wu, Y;Draisma, H;Wharton, J;Swietlik, EM;Cebola, I;Vasilaki, E;Balkhiyarova, Z;Jarvelin, MR;Auvinen, J;Herzig, KH;Coghlan, JG;Lordan, J;Church, C;Howard, LS;Pepke-Zaba, J;Toshner, M;Wort, SJ;Kiely, DG;Condliffe, R;Lawrie, A;Gräf, S;Morrell, NW;Wilkins, MR;Prokopenko, I;Rhodes, CJ;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. In vitro and In vivo evidence demonstrating chronic absence of Ref-1 Cysteine 65 impacts Ref-1 folding configuration, redox signaling, proliferation and metastasis in pancreatic cancer
    Authors: Mijit, M;Kpenu, E;Chowdhury, NN;Gampala, S;Wireman, R;Liu, S;Babb, O;Georgiadis, MM;Wan, J;Fishel, ML;Kelley, MR;
    Redox biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Cytokine-Induced iNOS in A549 Alveolar Epithelial Cells: A Potential Role in COVID-19 Lung Pathology
    Authors: Barilli, A;Recchia Luciani, G;Visigalli, R;Sala, R;Soli, M;Dall'Asta, V;Rotoli, BM;
    Biomedicines
    Species: Human
    Sample Types: Transfected Whole Cells, Whole Cells
    Applications: Bioassay
  5. An integrated organoid omics map extends modeling potential of kidney disease
    Authors: Lassé, M;El Saghir, J;Berthier, CC;Eddy, S;Fischer, M;Laufer, SD;Kylies, D;Hutzfeldt, A;Bonin, LL;Dumoulin, B;Menon, R;Vega-Warner, V;Eichinger, F;Alakwaa, F;Fermin, D;Billing, AM;Minakawa, A;McCown, PJ;Rose, MP;Godfrey, B;Meister, E;Wiech, T;Noriega, M;Chrysopoulou, M;Brandts, P;Ju, W;Reinhard, L;Hoxha, E;Grahammer, F;Lindenmeyer, MT;Huber, TB;Schlüter, H;Thiel, S;Mariani, LH;Puelles, VG;Braun, F;Kretzler, M;Demir, F;Harder, JL;Rinschen, MM;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Therapy-induced APOBEC3A drives evolution of persistent cancer cells
    Authors: Isozaki, H;Sakhtemani, R;Abbasi, A;Nikpour, N;Stanzione, M;Oh, S;Langenbucher, A;Monroe, S;Su, W;Cabanos, HF;Siddiqui, FM;Phan, N;Jalili, P;Timonina, D;Bilton, S;Gomez-Caraballo, M;Archibald, HL;Nangia, V;Dionne, K;Riley, A;Lawlor, M;Banwait, MK;Cobb, RG;Zou, L;Dyson, NJ;Ott, CJ;Benes, C;Getz, G;Chan, CS;Shaw, AT;Gainor, JF;Lin, JJ;Sequist, LV;Piotrowska, Z;Yeap, BY;Engelman, JA;Lee, JJ;Maruvka, YE;Buisson, R;Lawrence, MS;Hata, AN;
    Nature
    Species:  Human
    Sample Types: Whole Cells
    Applications: Immunofluorescence
  7. GPR84 regulates pulmonary inflammation by modulating neutrophil functions
    Authors: SW Wang, Q Zhang, D Lu, YC Fang, XC Yan, J Chen, ZK Xia, QT Yuan, LH Chen, YM Zhang, FJ Nan, X Xie
    Acta pharmacologica Sinica, 2023-04-04;0(0):1-11.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Lactobacillus rhamnosus postbiotic-induced immunomodulation as safer alternative to the use of live bacteria
    Authors: S Salva, I Tiscornia, F Gutiérrez, S Alvarez, M Bollati-Fo
    Cytokine, 2021-07-10;146(0):155631.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

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Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF
By Anonymous on 07/05/2024
Application: In vitro bioactivity in cell culture
In vitro bioactivity in cell culture Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF 10291-TA
Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF
By Anonymous on 06/15/2022
Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF 10291-TA
Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF
By Anonymous on 11/18/2020
Application: In vitro bioactivity in cell culture
Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF
By Jayalakshmi Miriyala on 10/01/2020
Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: I used this for the diffrentiation of monocytes to dendritic cell and it worked very well
Stem/Immune cell maintenance or differentiation Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF 10291-TA