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Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF

Catalog #: 862-VC
25 Citations 1 Review Datasheet / COA / SDS
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862-VC-100
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Recombinant Human VCAM‑1/CD106 Fc Chimera Protein SEC-MALS.
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Citations (25)
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), approximately 90-100% will adhere after 1 hour incubation at RT.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human VCAM-1/CD106 protein
Met Human VCAM-1
(Phe25-Glu698)
Accession # P19320		 IEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
P19320
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
101.6 kDa (monomer)
SDS-PAGE
115-130 kDa, reducing conditions

Product Datasheets

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862-VC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

862-VC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 week, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Human VCAM1/Fc/His (Catalog # 862-VC) has a molecular weight (MW) of 231.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VCAM-1/CD106

Human VCAM-1 (CD106, INCAM-110) is a cell adhesion molecule and a member of the immunoglobulin superfamily. Alternatively spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 amino acid extracellular segment that includes seven C2-type immunoglobulin domains. VCAM-1 is expressed by activated endothelial cells and certain other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs
7412 (Human); 22329 (Mouse)
Alternate Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

Citations for Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. The amoeboid migration of monocytes in confining channels requires the local remodeling of the cortical actin cytoskeleton by cofilin-1
    Authors: Ullo, MF;D'Amico, AE;Lavenus, SB;Logue, JS;
    Research square
    Species: Mouse
    Sample Types: Whole Cells, Transfected Whole Cells
    Applications: Bioassay
  2. The amoeboid migration of monocytes in confining channels requires the local remodeling of the cortical actin cytoskeleton by cofilin-1
    Authors: Ullo, MF;D'Amico, AE;Lavenus, SB;Logue, JS;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Dissecting the Therapeutic Mechanisms of Sphingosine-1-Phosphate Receptor Agonism during Ischaemia and Reperfusion
    Authors: Wilkins, GC;Gilmour, J;Giannoudaki, E;Kirby, JA;Sheerin, NS;Ali, S;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking
    Authors: Toffali, L;D'Ulivo, B;Giagulli, C;Montresor, A;Zenaro, E;Delledonne, M;Rossato, M;Iadarola, B;Sbarbati, A;Bernardi, P;Angelini, G;Rossi, B;Lopez, N;Linke, WA;Unger, A;Di Silvestre, D;Benazzi, L;De Palma, A;Motta, S;Constantin, G;Mauri, P;Laudanna, C;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. EZH1 repression generates mature iPSC-derived CAR T�cells with enhanced antitumor activity
    Authors: R Jing, I Scarfo, MA Najia, E Lummertz d, A Han, M Sanborn, T Bingham, C Kubaczka, DK Jha, M Falchetti, TM Schlaeger, TE North, MV Maus, GQ Daley
    Cell Stem Cell, 2022-08-04;29(8):1181-1196.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration
    Authors: M Mastrogiov, P Vargas, T Rose, C Cuche, E Esposito, M Juzans, H Laude, A Schneider, M Bernard, S Goyard, C Renaudat, MN Ungeheuer, J Delon, A Alcover, V Di Bartolo
    Science Advances, 2022-04-13;8(15):eabl5942.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. PI3Kgamma stimulates a high molecular weight form of myosin light chain kinase to promote myeloid cell adhesion and tumor inflammation
    Authors: MC Schmid, SW Kang, H Chen, M Paradise, A Ghebremedh, MM Kaneda, SM Chin, A Do, DM Watterson, JA Varner
    Nature Communications, 2022-04-01;13(1):1768.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Monocyte phenotyping and management of lipoprotein X syndrome
    Authors: Z Lian, A Saeed, X Peng, XD Perrard, X Jia, A Hussain, CM Ballantyne, H Wu
    J Clin Lipidol, 2020-09-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Sialyltransferase inhibition leads to inhibition of tumor cell interactions with E-selectin, VCAM1, and MADCAM1, and improves survival in a human multiple myeloma mouse model
    Authors: A Natoni, ML Farrell, S Harris, C Falank, L Kirkham-Mc, MS Macauley, MR Reagan, M O' Dwyer
    Haematologica, 2020-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
    Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
    J. Clin. Invest., 2019-05-14;129(7):2745-2759.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. ZAP70 expression enhances chemokine-driven chronic lymphocytic leukemia cell migration and arrest by valency regulation of integrins
    Authors: JM Laufer, R Lyck, DF Legler
    FASEB J., 2018-03-28;0(0):fj201701452RR.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, Cell Culture
  12. MiR221 promotes precursor B-cell retention in the bone marrow by amplifying the PI3K-signaling pathway in mice
    Authors: G Petkau, Y Kawano, I Wolf, M Knoll, F Melchers
    Eur. J. Immunol., 2018-03-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate
    Authors: PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley
    J. Leukoc. Biol., 2017-06-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Prolonged exposure to volatile anesthetic isoflurane worsens the outcome of polymicrobial abdominal sepsis
    Authors: Sophia Koutsogian
    Toxicol. Sci, 2017-04-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Kindlin-2 Modulates the Survival, Differentiation, and Migration of Induced Pluripotent Cell-Derived Mesenchymal Stromal Cells
    Authors: M Moslem, R Eggenschwi, C Wichmann, R Buhmann, T Cantz, R Henschler
    Stem Cells Int, 2017-01-09;2017(0):7316354.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Enhanced Biosensor Platforms for Detecting the Atherosclerotic Biomarker VCAM1 Based on Bioconjugation with Uniformly Oriented VCAM1-Targeting Nanobodies
    Authors: Peter Adriaensen
    Biosensors (Basel), 2016-07-05;6(3):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  17. Novel mutations in RASGRP2 encoding for CalDAG-GEFI abrogate Rap1 activation causing platelet dysfunction
    Authors: María Luisa Lozano
    Blood, 2016-05-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. The Neurorepellent Slit2 Inhibits Postadhesion Stabilization of Monocytes Tethered to Vascular Endothelial Cells.
    Authors: Mukovozov I, Huang Y, Zhang Q, Liu G, Siu A, Sokolskyy Y, Patel S, Hyduk S, Kutryk M, Cybulsky M, Robinson L
    J Immunol, 2015-08-21;195(7):3334-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET.
    Authors: Chakraborty S, Hu S, Wu S, Karmenyan A, Chiou A
    PLoS ONE, 2015-03-20;10(3):e0121399.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  20. Cytokine induction of VCAM-1 but not IL13Ralpha2 on glioma cells: a tale of two antibodies.
    Authors: Mahadev V, Starr R, Wright S, Martinez C, Jensen M, Barish M, Forman S, Brown C
    PLoS ONE, 2014-05-02;9(5):e95123.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Hydroxycarbamide decreases sickle reticulocyte adhesion to resting endothelium by inhibiting endothelial lutheran/basal cell adhesion molecule (Lu/BCAM) through phosphodiesterase 4A activation.
    Authors: Chaar V, Laurance S, Lapoumeroulie C, Cochet S, De Grandis M, Colin Y, Elion J, Le Van Kim C, El Nemer W
    J Biol Chem, 2014-03-10;289(16):11512-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Lutheran/basal cell adhesion molecule accelerates progression of crescentic glomerulonephritis in mice.
    Authors: Huang J, Filipe A, Rahuel C, Bonnin P, Mesnard L, Guerin C, Wang Y, Le Van Kim C, Colin Y, Tharaux P
    Kidney Int, 2014-01-15;85(5):1123-36.
    Species: Human
    Sample Types: Whole Blood
    Applications: Adhesion Assay
  23. A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling.
    Authors: Lee J, Sohn J, Ryu S, Hong C, Moon K, Park J
    J Cell Mol Med, 2013-07-16;17(10):1271-81.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  24. CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration.
    Authors: Chiang Y, Ho K, Sun C, Chiu J, Lee F, Liao F, Yang-Yen H, Yen J
    PLoS ONE, 2013-04-19;8(4):e61761.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces.
    Authors: Woolf E, Grigorova I, Sagiv A, Grabovsky V, Feigelson SW, Shulman Z, Hartmann T, Sixt M, Cyster JG, Alon R
    Nat. Immunol., 2007-08-26;8(10):1076-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF

Average Rating: 5 (Based on 1 Review)

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Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF
By Anonymous on 06/06/2018
Application: Cell Adhesion
Reason for Rating: the product works out very well in my cell adhesion assay and I got very nice dose curve.
Cell Adhesion Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF 862-VC