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Recombinant Human VCAM-1/CD106 Protein, CF

Catalog #: 809-VR
10 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
809-VR-050
809-VR-200
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Citations (10)
FAQs
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VCAM-1/CD106 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human VCAM-1/CD106 protein
Phe25-Glu698, with a substitution of Arg322Gly and an N-terminal Met
Accession #
P19320.1
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
74 kDa
SDS-PAGE
90-95 kDa, reducing conditions

Product Datasheets

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809-VR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

809-VR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Reconstitution Reconstitute at 1 mg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VCAM-1/CD106

Human VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN‑ gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7.

During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.

ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs
7412 (Human); 22329 (Mouse)
Alternate Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

Citations for Recombinant Human VCAM-1/CD106 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. In Vitro Study of the Multimodal Effect of Na+/K+ ATPase Blocker Ouabain on the Tumor Microenvironment and Malignant Cells
    Authors: Harich, OO;Gavriliuc, OI;Ordodi, VL;Tirziu, A;Paunescu, V;Panaitescu, C;Bojin, MF;
    Biomedicines
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Notch-IL21 signaling axis primes bone marrow T cell progenitor expansion
    Authors: K Sottoriva, NY Paik, Z White, T Bandara, L Shao, T Sano, K Pajcini
    JCI Insight, 2022-05-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Externalized histone H4 orchestrates chronic inflammation by inducing lytic cell death
    Authors: C Silvestre-, Q Braster, K Wichapong, EY Lee, JM Teulon, N Berrebeh, J Winter, JM Adrover, GS Santos, A Froese, P Lemnitzer, A Ortega-Góm, R Chevre, J Marschner, A Schumski, C Winter, L Perez-Oliv, C Pan, N Paulin, T Schoufour, H Hartwig, S González-R, F Kamp, RTA Megens, KA Mowen, M Gunzer, L Maegdefess, T Hackeng, E Lutgens, M Daemen, J von Blume, HJ Anders, VO Nikolaev, JL Pellequer, C Weber, A Hidalgo, GAF Nicolaes, GCL Wong, O Soehnlein
    Nature, 2019-05-01;569(7755):236-240.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Experimental identification and computational characterization of a novel extracellular metalloproteinase produced by Clostridium sordellii
    Authors: MJ Aldape, A Tao, DD Heeney, ER McIndoo, JM French, D Xu
    RSC Adv, 2017-03-02;7(23):13928-13938.
    Applications: Bioassay
  5. The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET.
    Authors: Chakraborty S, Hu S, Wu S, Karmenyan A, Chiou A
    PLoS ONE, 2015-03-20;10(3):e0121399.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  6. A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling.
    Authors: Lee J, Sohn J, Ryu S, Hong C, Moon K, Park J
    J Cell Mol Med, 2013-07-16;17(10):1271-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012-02-17;378(1):72-80.
    Applications: ELISA (Standard)
  8. CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
    Authors: Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E
    Leukemia, 2011-12-02;26(5):1098-105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009-07-30;51(4):734-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
    Authors: Arthos J, Cicala C, Martinelli E, MacLeod K, Van Ryk D, Wei D, Xiao Z, Veenstra TD, Conrad TP, Lempicki RA, McLaughlin S, Pascuccio M, Gopaul R, McNally J, Cruz CC, Censoplano N, Chung E, Reitano KN, Kottilil S, Goode DJ, Fauci AS
    Nat. Immunol., 2008-02-10;9(3):301-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human VCAM-1/CD106 Protein, CF

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Recombinant Human VCAM-1/CD106 Protein, CF
By Anonymous on 11/03/2017
Application: Immunoassay Standard

The VCAM antibody (AF809) was used as the capture antibody in a competitive ELISA assay. It was absorbed onto an ELISA plate. The competitor was recombinant VCAM (809-VR) that had been biotinylated. The calibrator curve also used recombinant VCAM (809-VR). Normal human serum and plasma samples were all quantifiable.

Immunoassay Standard Recombinant Human VCAM-1/CD106 Protein, CF 809-VR