Recombinant Human VE-Cadherin Protein His-tag, CF

Catalog #: 8440-VC Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8440-VC-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VE-Cadherin Protein His-tag, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is typically 20-100 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Human embryonic kidney cell, HEK293-derived human VE-Cadherin protein
Asp48-Gln599, with a C-terminal 6-His tag

Accession #

CAA56306

N-terminal Sequence
Analysis

Asp48

Structure / Form

Non-covalent dimer

Predicted Molecular Mass

63 kDa

SDS-PAGE

80-94 kDa, reducing conditions

Product Datasheets

8440-VC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8440-VC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: VE-Cadherin

VE-Cadherin, also known as Cadherin-5 and CD144, is a member of the atypical/type II subgroup of Cadherin homophilic adhesion proteins (1). Mature human
VE-Cadherin is a 125-135 kDa transmembrane glycoprotein that consistis of a 552 amino acid (aa) extracellular domain (ECD) with five Ca⁺⁺-binding Cadherin domains, a 21 aa transmembrane segment, and a 164 aa cytoplasmic domain (2, 3). Within the ECD, human VE-Cadherin shares approximately 75% aa sequence identity with mouse and rat VE-Cadherin. A 90 kDa portion of the VE-Cadherin ECD can be shed in a metalloproteinase-dependent mechanism and may function as a VE-Cadherin antagonist (4, 5). VE-Cadherin is expressed on the surface of vascular endothelial cells (EC) from early in embryogenesis through adulthood as well as in the placenta, on hematopoietic cell progenitors, and on a subpopulation of hematopoietic stem cells (6-8). It is a major protein component of adherens junctions between cells of the endothelium (9). VE-Cadherin supports the extension and stabilization of existing vascular sprouts (10). VE-Cadherin also regulates or is regulated by VEGF R2, type I and type II TGF-beta receptors, and other endothelial junction proteins such as JAM-C, Claudin-5, and N-Cadherin (11-15). Elevated levels of soluble VE-Cadherin are found in the serum of myocardial infarction, angina pectoris, rheumatoid arthritis, and colorectal cancer patients (16-18).

References

Giannotta, M. et al. (2013) Dev. Cell 26:441.
Breviario, F. et al. (1995) Arterioscler. Throm. Vasc. Biol. 15:1229.
Geyer, H. et al. (1999) Glycobiology 9:915.
Herren, B. et al. (1998) Mol. Biol. Cell 9:1589.
Li, H. et al. (2010) Cancer Gene Ther. 17:700.
Breier, G. et al. (1996) Blood 87:630.
Ema, M. et al. (2006) Blood 108:4018.
Oberlin, E. et al. (2010) Blood 116:4444.
Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.
Perryn, E.D. et al. (2008) Dev. Biol. 313:545.
Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
Orlova, V.V. et al. (2006) J. Exp. Med. 203:2703.
Taddei, A. et al. (2008) Nat. Cell Biol. 10:923.
Luo, Y. and G.L. Radice (2005) J. Cell Biol. 169:29.
Rudini, N. et al. (2008) EMBO J. 27:993.
Soeki, T. et al. (2004) Circ. J. 68:1.
Sulkowska, M. et al. (2006) Tumori 92:67.
Sidibe, A. et al. (2012) Arthritis Rheum. 64:77.

Long Name

Vascular Endothelium Cadherin

Entrez Gene IDs

1003 (Human); 12562 (Mouse)

Alternate Names

7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin