Recombinant Human VSIG3 Fc Chimera Protein, CF Summary
Product Specifications
Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) binds to Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) with an ED50 of 0.0750 - 0.750 μg/mL.
| Human VSIG3 (Leu23-Gly245) Accession # BAC07546 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9229-VS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with trehalose. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human VSIG3 Fc Chimera Protein (Catalog # 9229-VS) binds to Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (7126-B7) with an ED50 of 0.0750 - 0.750 μg/mL.
Reconstitution Calculator
Background: VSIG3
VSIG3, also known as IGSF11, BT-IgSF, and CLMP, is an approximately 50 kDa transmembrane adhesion protein (1). Mature human VSIG3 consists of a 219 amino acid (aa) extracellular domain (ECD) that contains two tandem Ig-like domains, a 21 aa transmembrane segment, and a 169 aa cytoplasmic domain (2). Within the ECD, human VSIG3 shares 95% aa sequence identity with mouse and rat VSIG3. Alternative splicing generates additional isoforms with a substituted signal peptide that may also have a deletion in the second Ig-like domain (3). VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (2-4). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (3-5). VSIG3 also localizes to neuronal postsynaptic densisties where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (6). The short isoform can be up-regulated in gastric cancer (7). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (8).
- Schreiber, J. et al. (2014) Adv. Neurobiol. 8:21.
- Suzu, S. et al. (2002) Biochem. Biophys. Res. Commun. 296:1215.
- Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
- Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
- Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
- Jang, S. et al. (2016) Nat. Neurosci. 19:84.
- Watanabe, T. et al. (2005) Cancer Sci. 96:498.
- Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.
Citations for Recombinant Human VSIG3 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Structure and Functional Binding Epitope of V-domain Ig Suppressor of T Cell Activation
Authors: N Mehta, S Maddineni, II Mathews, R Andres Par, PS Huang, JR Cochran
Cell Rep, 2019-09-03;28(10):2509-2516.e5.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Capture -
VSIG-3 as a ligand of VISTA inhibits human T-cell function.
Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
Immunology, 2018-10-10;156(1):74-85.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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0.5 ug VSIG3-hIgG1 (IGSF11) or hIgG1 were added to cells expressing hVISTA or a negative control. Binding was assessed using an anti-human 488 secondary antibody.
