Home / Wnt/RSPO2 / Recombinant Human Wnt/RSPO2 Agonist Protein, CF

Recombinant Human Wnt/RSPO2 Agonist Protein, CF

Catalog #: BT-WRSP2 Datasheet / COA / SDS

Improve your stem cell and organoid cultures with our New Wnt/R-Spondin Agonist Proteins (Catalog # BT-WRSP1, # BT-WRSP2, and # BT-WRSP3). These proteins exhibit similar or higher activity than the individual Wnt-3a and R-Spondin proteins together, simplifying experiments and providing time and cost savings. See the data in this Application Note. 

Show More
Catalog # Availability Size / Price Qty
BT-WRSP2-050 In Stock50 ug / $499
BT-WRSP2-100 In Stock100 ug / $899
BT-WRSP2-500 In Stock500 ug / $3,399

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

Bulk Orders
Recombinant Human Wnt/RSPO2 Agonist Protein Bioactivity.
2 Images
Product Details
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background

Recombinant Human Wnt/RSPO2 Agonist Protein, CF Summary

Product Specifications

Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 6.00‑72.0 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt/RSPO2 protein
Proprietary
Structure / Form
Disulfide-linked homodimer
SDS-PAGE
83-97 kDa & >190 kDa under reducing conditions.

Product Datasheets

You must select a language.

x

BT-WRSP2

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-WRSP2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Human Wnt/RSPO2 Agonist Protein (Catalog # BT-WRSP2) activates TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 6.00‑72.0 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human Wnt/RSPO2 Agonist Protein (Catalog # BT-WRSP2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-97 & >190 kDa under reducing conditions.

Reconstitution Calculator

Background: Wnt/RSPO2

Wnt signaling is known to promote self-renewal of adult stem cells. R-Spondins amplify Wnt signaling in adult stem cells by binding to LGR5 receptors resulting in increased levels of Wnt receptor localization at the membrane surface. Many current organoid protocols include addition of Wnt-3a and R-Spondin proteins to the media for optimal adult stem cell maintenance and health of organoids. Fusion proteins known known as Wnt Surrogates have been described that have enhanced solubility when compared to the widely used Wnt-3a protein and mirror the activities of Wnt signaling.1 R&D Systems has constructed recombinant fusion proteins designed to bind elements of the Wnt receptor system including LGR-5, Frizzled, and LRP-5/6. These can initiate canonical Wnt signaling and are designed to simplify cell culture protocols by substituting for conditioned media or the addition of recombinant Wnt and R-Spondin.

References
  1. Janda et al. (2017) Nature 545:234.
Long Name
Wnt/RSPO2
Entrez Gene IDs
340419 (Human); 239405 (Mouse); 500863 (Rat)
Alternate Names
CRISTIN2; HHRRD; roof plate-specific spondin-2; R-spondin 2 homolog; R-spondin-2; TETAMS2; Wnt; Wnt/RSPO2

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Wnt/RSPO2 Agonist Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Wnt/RSPO2 Agonist Protein, CF and earn rewards!

Have you used Recombinant Human Wnt/RSPO2 Agonist Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review