Recombinant Mouse Active Heparanase/HPSE Protein, CF

Recombinant Mouse Active Heparanase/HPSE Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Assay Procedure

Background: Heparanase/HPSE

Heparanase (HPSE) selectively cleaves heparan sulfate (HS) at specific sites on HS proteoglycans (HSPGs) (1, 2, 3, 4). The enzyme is synthesized as an inactive 65 kDa proenzyme that is secreted via the Golgi apparatus and associates with the cell membrane through interaction with HSPGs (5). It is then endocytosed and transferred to lysosomes (6) where cathepsin L activates it by removing an internal inhibitory peptide, forming a heterodimer composed of an 8 kDa and a 50 kDa subunit (7, 8). Under certain stimuli, the active enzyme is transferred back to the cell surface, where it participates in extracellular matrix degradation and remodeling (9). HPSE facilitates cell migration associated with metastasis, wound healing and inflammation (10). An increase in its activity is associated with an increase in VEGF activity, which further enhances angiogenesis (11). HPSE also enhances shedding of syndecans and increases endothelial invasion and angiogenesis in myelomas (12). It acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII (13). In addition, it increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity (14). HPSE is highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues (15). Mouse HPSE shows 76% identity to human HPSE at amino acid sequence. The enzyme activity of recombinant mouse HPSE was assayed using recombinant syndecan 4 that was biotinylated at the non-reducing end of its HS chains (catalogue ES020) in ELISA format (16).

Manufacturing Specifications

1. Vlodavsky, I. et al. (1999) Nat. Med. 5:793.
2. Hulett, M.D. et al. (1999) Nat. Med. 5:803.
3. Gong, F. et al. (2003) J. Biol. Chem. 278:35152.
4. Peterson, S.B. and Liu, J. (2010) J. Biol. Chem. 285:14504.
5. Nadav L. et al. (2002) J. Cell Sci. 115:2179.
6. Gingis-Velitski, S. et al. (2004) J. Biol. Chem. 279:44084.
7. Abboud-Jarrous, G. et al. (2008) J. Biol. Chem. 283:18167.
8. Zetser, A. et al. (2004) J. Cell Sci. 117:2249.
9. Zcharia E. et al. (2001) J. Mammary Gland Biol. Neoplasia 6:311.
10. Fux, L. et al. (2009) Trends Biochem. Sci. 34:511.
11. Cohen-Kaplan, V. et al. (2008) Int. J. Cancer 123:2566.
12. Purushothaman, A. et al. (2010) Blood 115:2449.
13. Nadir, Y. et al. (2010) Haematologica 95:1927.
14. Goldshmidt, O. et al. (2003) FASEB J. 17:1015.
15. Kussie, P.H. et al. (1999) Biochem. Biophys. Res. Commun. 261:183.
16. Wu, Z.L. et al. (2017) Glycobiology 27:518.

Related Research Areas

• Cancer Biomarkers
• Coagulation
• Glycobiology-related Enzymes
• Lysosomal Enzymes
• Proteoglycan Regulators
• Search Bioprocessing Enzymes
• Search Glycobiology-related Enzymes