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Recombinant Mouse APRIL/TNFSF13 Protein

Catalog #: 7907-AP
4 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
7907-AP-010/CF

With Carrier

Catalog # Availability Size / Price Qty
7907-AP-010
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Product Details
Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse APRIL/TNFSF13 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.5-3 ng/mL in the presence of a cross‑linking antibody, Mouse Anti‑Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).
Source
Chinese Hamster Ovary cell line, CHO-derived mouse APRIL/TNFSF13 protein
HA
(YPYDVPDYA)
 GCN4-IZ GGGSGGGSGGGS  Mouse APRIL/TNFSF13
(Ala96-Leu240)
Accession # NP_001152977
N-terminus C-terminus
Accession #
NP_001152977
N-terminal Sequence
Analysis
Tyr
Predicted Molecular Mass
21.8 kDa
SDS-PAGE
23-30 kDa, reducing conditions

Product Datasheets

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7907-AP (with carrier)

Product Datasheet
COA
SDS

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7907-AP/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7907-AP

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7907-AP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 100 μg/mL in deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APRIL/TNFSF13

APRIL (a proliferation‑inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily (1). It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule (2‑4). Secreted mouse APRIL, which consists almost entirely of a single TNF homology domain, shares 85% and 95% amino acid sequence identity with human and rat APRIL, respectively (2, 3). Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R (2, 5, 6). APRIL binds to heparan sulfate proteoglycans (HSPGs) independently of its binding to TACI and BCMA (6, 7). The interaction with HSPGs induces APRIL oligomerization, and this augments TACI‑, or BMCA‑mediated effects (7, 8). HSPGs are also critical for the tumor growth‑promoting effects attributed to APRIL (6). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (10). TWE‑PRIL is a bioactive hybrid protein produced by gene splicing. It consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (11). TWE‑PRIL is expressed in monocytes and activated T cells and, in contrast to APRIL, is presented on the cell surface (11). APRIL enhances the proliferation and survival of plasma cells and also promotes T cell‑dependent humoral responses (2, 12, 13). In the context of autoimmune disorders, however, APRIL can inhibit pathologic humoral responses as well as disease progression (14). Its expression by CD4+ T cells inhibits the production of Th2 cytokines and allergic inflammation (15). APRIL levels are elevated in the serum during coronary artery disease (16), and it is also elevated in many cancers primarily due to expression by tumor‑infiltrating neutrophils (4, 7, 17).

References
  1. Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
  2. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  3. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
  4. Kelly, K. et al. (2000) Cancer Res. 60:1021.
  5. Day, E.S. et al. (2005) Biochemistry 44:1919.
  6. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
  7. Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
  8. Schwaller, J. et al. (2007) Blood 109:331.
  9. Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
  10. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  11. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.
  12. Benson, M.J. et al. (2008) J. Immunol. 180:3655.
  13. He, B. et al. (2004) J. Immunol. 172:3268.
  14. Fernandez, L. et al. (2013) Ann. Rheum. Dis. Epub Nov 24. PMID 23178293.
  15. Xiao, Y. et al. (2011) Eur. J. Immunol. 41:164.
  16. Sandberg, W.J. et al. (2009) Thromb. Haemost. 102:704.
  17. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
Long Name
A Proliferation-inducing Ligand
Entrez Gene IDs
8741 (Human); 69583 (Mouse); 287437 (Rat)
Alternate Names
A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2

Citations for Recombinant Mouse APRIL/TNFSF13 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. BAFF and APRIL counterregulate susceptibility to inflammation-induced preterm birth
    Authors: JR Doll, ME Moreno-Fer, TE Stankiewic, JL Wayland, A Wilburn, B Weinhaus, CA Chougnet, D Giordano, M Cappellett, P Presicce, SG Kallapur, N Salomonis, T Tilburgs, S Divanovic
    Cell Reports, 2023-04-05;42(4):112352.
    Species: Mouse
    Sample Types:
    Applications: In vivo assay
  2. A BAFF/APRIL axis regulates obesogenic diet-driven weight gain
    Authors: CC Chan, ITW Harley, PT Pfluger, A Trompette, TE Stankiewic, JL Allen, ME Moreno-Fer, MSMA Damen, JR Oates, PC Alarcon, JR Doll, MJ Flick, LM Flick, J Sanchez-Gu, R Mukherjee, R Karns, M Helmrath, TH Inge, SP Weisberg, SJ Pamp, DA Relman, RJ Seeley, MH Tschöp, CL Karp, S Divanovic
    Nature Communications, 2021-05-18;12(1):2911.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. TACI expression and plasma cell differentiation are impaired in the absence of functional I?BNS
    Authors: S Khoenkhoen, E Erikson, M Ádori, JM Stark, JL Scholz, MP Cancro, GK Pedersen, GB Karlsson H
    Immunol. Cell Biol., 2019-01-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2015-07-03;30(1):163-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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