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Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein, CF

Catalog #: 10336-BL Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10336-BL-050
Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein (Catalog # 10336-BL) is immobilized at 5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein that produces 50% of the optimal binding response is found to be approximately 4‑20 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse BLAME/SLAMF8 protein
Mouse BLAME/SLAMF8
(Val21-Asp231)
Accession # NP_083360.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
NP_083360.2
N-terminal Sequence
Analysis
Val21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa
SDS-PAGE
52-70 kDa, under reducing conditions

Product Datasheets

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10336-BL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10336-BL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein Binding Activity View Larger

When Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein (Catalog # 10336-BL) is immobilized at 5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein that produces 50% of the optimal binding response is found to be approximately 4-20 μg/mL.

SDS-PAGE Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse BLAME/SLAMF8 Fc Chimera (Catalog # 10336-BL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-70 kDa and 100-140 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BLAME/SLAMF8

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). It is expressed on dendritic cells and IFN-gamma stimulated monocytes. Over-expression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3, 4). Mature mouse BLAME consists of a 211 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 26 aa cytoplasmic domain. Within the ECD, mouse BLAME shares 79% and 95% aa sequence identity with human and rat BLAME, respectively.

References
  1. McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
  2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
  3. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.
  4. Wang, G. et al. (2012) J. Immunol. 188:5829.
Long Name
SLAM Family Member 8
Entrez Gene IDs
56833 (Human); 74748 (Mouse); 289237 (Rat); 102122568 (Cynomolgus Monkey)
Alternate Names
B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; BLAME; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8

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