Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF Summary
Product Specifications
Met1-Gly693, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6768-CA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Cadherin-13
Cadherin-13, also known as T-Cadherin and H‑Cadherin, is a GPI-anchored protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Following removal of its C‑terminal propeptide, Cadherin-13 is expressed on the cell surface both with and without its N-terminal propeptide in species of approximately 120 kDa and 100 kDa, respectively (3, 4). Mouse Cadherin-13 contains five tandem Cadherin repeats and shares 95% and 98% aa sequence identity with human and rat Cadherin-13, respectively (5). It is most highly expressed in the circulatory and nervous systems, particularly on cardiac myocytes, vascular endothelial and smooth muscle cells, and neurons and astrocytes in the brain (3, 4, 6 - 9). Cadherin-13 exerts a negative influence on neurite extension, and it is down‑regulated in neurons upon NGF stimulation (10, 11). Homotypic Cadherin-13 interactions promote intercellular adhesion which can be inhibited by its binding to LDL (12). Cadherin-13 also mediates the cardioprotective effects of Adiponectin by directly binding Adiponectin (selectively the hexameric and HMW forms) and trapping it on vascular endothelial cells and cardiomyocytes (6 ‑ 8). The role of Cadherin-13 in the vasculature is complex. When expressed on vascular endothelial cells it promotes angiogenesis, but when expressed on stromal cells it inhibits neovascularization (13, 14). Its down‑regulation on breast carcinoma cells and up‑regulation on the vasculature of various tumors limits tumor cell proliferation and angiogenesis but also enhances tumor progression (4, 8, 15).
- Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
- Philippova, M. et al. (2009) Cell. Signal. 21:1035.
- Stambolsky, D.V. et al. (1999) Biochim. Biophys. Acta 1416:155.
- Doyle, D.D. et al. (1998) J. Biol. Chem. 273:6937.
- Wyder, L. et al. (2000) Cancer Res. 60:4682.
- Denzel, M.S. et al. (2010) J. Clin. Invest. 120:4342.
- Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
- Hebbard, L.W. et al. (2008) Cancer Res. 68:1407.
- Takeuchi, T. et al. (2000) J. Neurochem. 74:1489.
- Fredette, B.J. et al. (1996) Development 122:3163.
- Bai, S. et al. (2007) J. Biol. Chem. 282:27171.
- Resink, T.J. et al. (1999) FEBS Lett. 463:29.
- Philippova, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26:2222.
- Rubina, K. et al. (2007) Angiogenesis 10:183.
- Lee, S.W. (1996) Nat. Med. 2:776.
Citation for Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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CDH13 abundance interferes with adipocyte differentiation and is a novel biomarker for adipose tissue health
Authors: S Göddeke, B Knebel, P Fahlbusch, T Hörbelt, G Poschmann, F van de Vel, T Benninghof, H Al-Hasani, S Jacob, Y Van Nieuwe, B Lapauw, S Lehr, DM Ouwens, J Kotzka
Int J Obes (Lond), 2018-02-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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