Recombinant Mouse Chitinase 3-like 1/YKL-40 Protein, CF
Recombinant Mouse Chitinase 3-like 1/YKL-40 Protein, CF Summary
Product Specifications
Optimal concentrations should be determined by each laboratory for each application.
Tyr22-Ala381, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2649-CH
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Chitinase 3-like 1/YKL-40
Chitinase 3-like 1 (CHI3L1), also called breast regression protein 39 (BRP39) in mouse, or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1 ‑ 4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5 ‑ 7). The mouse CHI3L1 cDNA encodes 381 amino acids (aa), including a 21 aa signal sequence and a 360 aa mature region with two intermolecular disulfides (3). Mature mouse CHI3L1 shares 73%, 75%, 72%, 71%, 70% and 69% aa sequence identity with human, rat, equine, porcine, canine and bovine CHI3L1, respectively. CHI3L1 does not show chitotriosidase activity, but binds chitin and is thus termed a chi-lectin (1 ‑ 4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6 ‑ 9). Human elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9 ‑ 12). Human single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15). Mice lacking CHI3L1 have markedly diminished antigen-induced Th2 responses, which can be rescued by expression of human CHI3L1 (16).
- Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
- Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
- Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
- Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
- Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
- Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
- Shao, R. et al. (2009) Oncogene 28:4456.
- Recklies, A.D. et al. (2002) Biochem. J. 365:119.
- Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
- Letuve, S. et al. (2008) J Immunol. 181:5167.
- Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
- Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
- Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
- Johansen, J.S. et al. (1999) Rheumatology 38:618.
- Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
- Lee, C.G. et al. (2009) J. Exp. Med. 206:1149.
Citations for Recombinant Mouse Chitinase 3-like 1/YKL-40 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Inhibition of Amyloid-? (A?)-Induced Cognitive Impairment and Neuroinflammation in CHI3L1 Knockout Mice through Downregulation of ERK-PTX3 Pathway
Authors: Ham, HJ;Lee, YS;Koo, JK;Yun, J;Son, DJ;Han, SB;Hong, JT;
International journal of molecular sciences
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Chitinase 3-like 1 is neurotoxic in primary cultured neurons
Authors: C Matute-Bla, L Calvo-Barr, I Carballo-C, R Gonzalo, A Sanchez, M Vila, X Montalban, M Comabella
Sci Rep, 2020-04-28;10(1):7118.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL-13R?2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses
Nat Commun, 2016-09-15;7(0):12752.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Antigen-specific over-expression of human cartilage glycoprotein 39 on CD4+ CD25+ forkhead box protein 3+ regulatory T cells in the generation of glucose-6-phosphate isomerase-induced arthritis.
Authors: Tanaka Y, Matsumoto I, Inoue A, Umeda N, Takai C, Sumida T
Clin Exp Immunol, 2014-08-01;177(2):419-27.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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