Recombinant Mouse CKAP4/p63 Protein, CF

Recombinant Mouse CKAP4/p63 Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CKAP4/p63

CKAP4 (Cytoskeleton-associated protein 4; also CLIMP63 and p63) is a 63-64 kDa molecule that belongs to no known protein family. It is found intracellularly, and on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells (1). CKAP4 has a bimodal distribution. First, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus (2). This localization is dependent upon its ability to form homooligomers, and its presence serves to anchor microtubules and direct the formation of tubular ER. Second, it is embedded in the plasma membrane and serves as a receptor for SP‑A/surfactant protein-A (in lung) (2) and tPA (in vessels) (3). Mouse CKAP4 is a 575 amino acid (aa) type II transmembrane nonglycosylated protein. It contains an 85 aa N-terminal cytoplasmic region plus a 467 aa C-terminal luminal domain (aa 109-575) (4). The luminal domain contains three coiled-coil regions (aa 125-193; 236‑438; 507-575) plus three utilized phosphorylation sites (5). CKAP4 undergoes reversible palmitoylation (6). Over aa 109-575, mouse CKAP4 shares 82% and 94% aa sequence identity with human and rat CKAP4, respectively. CKAP4 has been shown to be required for APF-mediated signaling (6-9), for gentamycin-induced cytotoxicity (8), for regulation of tPA function (3), and for SP-A-induced surfactant uptake by pneumocytes (10). CKAP4 interacts with Dickkopf-1 (Dkk-1) to promote activation of AKT-dependent, Wnt-independent signaling(11). Both CKAP4 and Dkk-1 are frequently up-regulated in pancreatic and lung cancers (11). Dkk-1 binds to CKAP4 with high affinity that is comparable to but independent of its interaction with LRP5/6 (11).

Related Research Areas

• Cancer Biomarkers