Recombinant Mouse Follistatin-like 1 Protein, CF
Recombinant Mouse Follistatin-like 1 Protein, CF Summary
Product Specifications
Glu19-Ile306, with a C-terminal Asp-Ile and 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1738-FN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Follistatin-like 1/FSTL1
Follistatin-like 1 (FSTL1 or FSL1), also known as FRP (follistatin-related protein), Flik (follistatin-like), and TSC-36 (TGF-beta 1-stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM-40/SPARC/Osteonectin family (1-3). The mouse FSTL1 cDNA encodes 306 amino acids (aa), including an 18 aa signal sequence, a cysteine-rich Follistatin (EGF- and kazal-like) domain, two apparently nonfunctional EF-hand calcium-binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature mouse FSTL1 shares 94%, 96%, 94%, 93% and 93% aa identity with human, rat, equine, bovine and canine FSTL1, respectively. FSTL1 was first identified as a TGF-beta -induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In vitro, FSTL1 interacts directly with BMP-4, while in vivo, it regulates BMP-4 signaling during normal lung development, as demonstrated by lethal respiratory failure in mice deleted for FSTL1 (6). In humans, FSTL1 is a common rheumatoid arthritis autoantigen (2). It is reported to be either proinflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (7-10). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (11, 12). Cardiac and circulating FSTL1 is generally increased in conditions such as heart failure and acute coronary syndrome (12-14). FSTL1 also appears to be a tumor suppressor, showing down‑regulated expression in many human cancers (4, 15, 16). In vitro, it slows proliferation and MMP-dependent migration, and increases FAS-dependent apoptosis of tumor cell lines (15).
- Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
- Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
- Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
- Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
- Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
- Geng, Y et al. (2011) Proc. Natl. Acad. Sci. USA 108:7058.
- Clutter, S.D. et al. (2009) J. Immunol. 182:234.
- Miyamae, T. et al. (2006) J. Immunol. 177:4758.
- Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
- Tanaka, M. et al. (2003) Int. Immunol. 15:71.
- Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
- Oshima, Y. et al. (2008) Circulation 117:3099.
- Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
- Widera, C. et al. (2009) Clin. Chem. 55:1794.
- Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
- Mashimo, J. et al. (1997) Cancer Lett. 113:213.
Citations for Recombinant Mouse Follistatin-like 1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Cell networks in the mouse liver during partial hepatectomy
Authors: Li, B;Rodrigo-Torres, D;Pelz, C;Innes, B;Canaday, P;Chai, S;Zandstra, P;Bader, GD;Grompe, M;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Immune stimulation recruits a subset of pro-regenerative macrophages to the retina that promotes axonal regrowth of injured neurons
Authors: Andries, L;Kancheva, D;Masin, L;Scheyltjens, I;Van Hove, H;De Vlaminck, K;Bergmans, S;Claes, M;De Groef, L;Moons, L;Movahedi, K;
Acta neuropathologica communications
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/beta-catenin signaling in obstructed kidneys in vivo
Authors: Y Zhang, Y Wang, G Zheng, Y Liu, J Li, H Huang, C Xu, Y Zeng, X Zhang, J Qin, C Dai, HO Hambrock, U Hartmann, B Feng, KK Mak, Y Liu, HY Lan, Y Huang, ZH Zheng, Y Xia
The Journal of Biological Chemistry, 2022-05-04;0(0):102010.
Species: Mouse
Sample Types: Whole Cell
Applications: Cell Culture -
Ups and downs: the PPARgamma/p-PPARgamma seesaw of follistatin-like 1 and integrin receptor signaling in adipogenesis
Authors: D Fang, X Shi, X Jia, C Yang, L Wang, B Du, T Lu, L Shan, Y Gao
Molecular Metabolism, 2021-11-20;0(0):101400.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
BBS4 regulates the expression and secretion of FSTL1, a protein that participates in ciliogenesis and the differentiation of 3T3-L1
Authors: V Prieto-Ech, S Lodh, L Colman, N Bobba, L Santos, N Katsanis, C Escande, NA Zaghloul, JL Badano
Sci Rep, 2017-08-29;7(1):9765.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer
Authors: JS Lim, A Ibaseta, MM Fischer, B Cancilla, G O'Young, S Cristea, VC Luca, D Yang, NS Jahchan, C Hamard, M Antoine, M Wislez, C Kong, J Cain, YW Liu, AM Kapoun, KC Garcia, T Hoey, CL Murriel, J Sage
Nature, 2017-05-10;545(7654):360-364.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Follistatin-like 1: a potential mediator of inflammation in obesity.
Authors: Fan N, Sun H, Wang Y, Wang Y, Zhang L, Xia Z, Peng L, Hou Y, Shen W, Liu R, Yin J, Peng Y
Mediators Inflamm, 2013-11-21;2013(0):752519.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay
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