Recombinant Mouse FOLR1 Protein, CF Summary
Product Specifications
When Folic Acid-Bovine Serum Albumin is immobilized at 5 μg/mL (100 μL/well), Recombinant Mouse (rm) FOLR1 inhibits 50% binding of biotinylated rmFOLR1 (7.9 nM) at the concentration range of 30-120 nM.
Met1-Ser232, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6936-FR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: FOLR1
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3 - 7). Mouse FOLR1 shares 83% and 93% amino acid sequence identity with human and rat FOLR1, respectively. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas (8, 9). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (10). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 11). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (12).
- Kelemen, L.E. (2006) Int. J. Cancer 119:243.
- Fowler, B. et al. (2001) Kidney Int. 59:S-221.
- Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
- Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
- Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
- Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
- Brigle, K.E. et al. (1991) J. Biol. Chem. 266:17243.
- Ross, J.F. et al. (1994) Cancer 73:2432.
- Parker, N. et al. (2005) Anal. Biochem. 338:284.
- Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
- Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
- Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse FOLR1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse FOLR1 Protein, CF and earn rewards!
Have you used Recombinant Mouse FOLR1 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
