Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF
Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF Summary
Product Specifications
Gln36-Leu288, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10516-MS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
2 μg/lane of Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein (Catalog # 10516-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 29-45 kDa.
Reconstitution Calculator
Background: Mesothelin
Mesothelin N-terminal, commonly known as Megakaryocyte Potentiating Factor (MPF), is a 31 kDa soluble protein that was originally identified in an PHC-Y5 culture system where it exhibited megakaryocyte potentiating activity (1). MPF is closely related to mesothelin (MSLN), a 40 kDa glycosylphosphatidyl inositol (GPI)-linked protein that is highly overexpressed in various types of cancer, such as mesothelioma, ovarian and pancreatic cancers (2). Both MPF and MSLN originate from a common 71 kDa precursor protein that harbors a furin cleavage site between amino acid residues 288-293. The 31 kDa soluble N-terminal MPF fragment is secreted into blood and the C-terminal 40 kDa fragment remains membrane-bound and classified as mature mesothelin (3). Mouse MPF is composed of residues 36-288 from the precursor protein and contains one N-linked glycosylation site (N93) and one phosphorylated serine (S202). Mouse MPF shares 56% and 84% sequence identity with human and rat MPF, respectively. It is expressed mainly in the heart and lungs (4) and can be upregulated in the presence of Wnt-1 (5). MSLN was shown to be capable of binding to cancer antigen 125/mucin16 (CA125/MUC16). Apart from its role as a megakaryocyte potentiating cytokine, MPF may have the potential to serve as a biomarker for detection and monitoring of malignant mesothelioma and related cancers (6).
- Yamaguchi, N. et al. (1994) J. Biol. Chem. 260: 22:805.
- Hassan R. et al. (2004) Clin. Cancer Res. 10:3937.
- Shiomi, K. et al. (2006) Cancer. Sci. 97:928.
- Bera T.K. Pastan I. (2000) Mol. Cell. Biol. 20:2902.
- Prieve M.G. Moon R.T. (2003) BMC Dev Biol. 3:2.
- Raiko, I. et al. (2017) Biochem. Biophy. Res. Com. 486:526.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF and earn rewards!
Have you used Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
