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Recombinant Mouse MIF Protein

Catalog #: 1978-MF
16 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
1978-MF-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1978-MF-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (16)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse MIF Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
E. coli-derived mouse MIF protein
Pro2-Ala115
Accession #
NP_034928
Predicted Molecular Mass
12.4 kDa

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1978-MF (with carrier)

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1978-MF/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1978-MF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1978-MF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIF

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Mouse MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs nonclassically via an ABCA1 transporter (6). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 8). Structure-function studies suggests MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol- to-keto) has been demonstrated and is dependent upon Pro at position #1 (9). Amino acids 3-23 have also been shown to be reminescent of a GST glutathione-binding domain (10). MIF has proinflammatory cytokine activity centered on aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates, NO production and TNF-alpha release following IFN-gamma activation (11, 12). Mouse MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (13, 14). Mouse MIF is active on human cells, while human MIF is active on mouse cells (12). Mouse MIF is 99%, 84%, 90%, and 90% aa identical to rat, porcine, bovine and human MIF, respectively.

References
  1. Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12. 
  2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1. 
  3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791. 
  4. Bozza, M. et al. (1995) Genomics 27:412. 
  5. Mitchell, R. et al. (1995) J. Immunol. 154:3863. 
  6. Flieger, O. et al. (2003) FEBS Lett. 551:78. 
  7. Lanahan, A. et al. (1992) Mol. Cell. Biol. 12:3919. 
  8. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
  9. Stamps, S.L. et al. (2000) Biochemistry 39:9671.
  10. Blocki, F.A. et al. (1993) Protein Sci. 2:2095.
  11. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
  12. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
  13. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  14. Meyer-Siegler, K.L. and P.L. Vera  (2005) J. Urology 173:615.
Long Name
Macrophage Migration Inhibitory Factor
Entrez Gene IDs
4282 (Human); 17319 (Mouse)
Alternate Names
EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase

Citations for Recombinant Mouse MIF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Involvement of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor in proinflammatory mechanisms promoting cardiovascular injury mechanisms promoting cardiovascular inflammation tThe combined action of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor increases proinflammatory mediator production by cardiomyocytes and vascular endothelial cells
    Authors: CS Rigazio, N Mariz-Pont, EP Caballero, FN Penas, NB Goren, MH Santamaría, RS Corral
    Microbial pathogenesis, 2022-11-12;0(0):105881.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Phenotyping of circulating extracellular vesicles (EVs) in obesity identifies large EVs as functional conveyors of Macrophage Migration Inhibitory Factor
    Authors: J Amosse, M Durcin, M Malloci, L Vergori, A Fleury, F Gagnadoux, S Dubois, G Simard, J Boursier, O Hue, MC Martinez, R Andriantsi, S Le Lay
    Mol Metab, 2018-10-09;18(0):134-142.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis
    Authors: N Priego, L Zhu, C Monteiro, M Mulders, D Wasilewski, W Bindeman, L Doglio, L Martínez, E Martínez-S, SRY Cajal, D Megías, E Hernández-, C Blanco-Apa, L Martínez, E Zarzuela, J Muñoz, C Fustero-To, E Pineiro, A Hernández-, L Bertero, V Poli, M Sánchez-Ma, JA Menendez, R Soffietti, J Bosch-Barr, M Valiente
    Nat. Med., 2018-06-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Novel half-life extended anti-MIF nanobodies protect against endotoxic shock
    Authors: A Sparkes, P De Baetsel, L Brys, I Cabrito, YG Sterckx, S Schoonoogh, S Muylderman, G Raes, R Bucala, P Vanlandsch, JA Van Ginder, B Stijlemans
    FASEB J., 2018-01-25;0(0):fj201701189R.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  5. CHD7 promotes proliferation of neural stem cells mediated by MIF
    Mol Brain, 2016-12-13;9(1):96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Relationship between elevated soluble CD74 and severity of experimental and clinical ALI/ARDS
    Sci Rep, 2016-07-22;6(0):30067.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Macrophage Migration Inhibitor Factor Upregulates MCP-1 Expression in an Autocrine Manner in Hepatocytes during Acute Mouse Liver Injury.
    Authors: Jieshi Xie, Le Yang, Lei Tian, Weiyang Li, Lin Yang, Liying Li
    Scientific Reports, 2016-06-08;0(0):2045-2322.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion
    Stem Cells, 2016-05-27;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Macrophage migration inhibitory factor promotes cardiac stem cell proliferation and endothelial differentiation through the activation of the PI3K/Akt/mTOR and AMPK pathways
    Authors: J Cui, F Zhang, Y Wang, J Liu, X Ming, J Hou, B Lv, S Fang, B Yu
    Int J Mol Med, 2016-03-29;37(5):1299-309.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. The critical role of macrophage migration inhibitory factor in insulin activity.
    Authors: Vujicic M, Senerovic L, Nikolic I, Saksida T, Stosic-Grujicic S, Stojanovic I
    Cytokine, 2014-06-04;69(1):39-46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Review: putative roles for the macrophage migratory inhibitory factor at the maternal fetal interface.
    Authors: Bevilacqua E, Paulesu L, Ferro E, Ietta F, Faria M, Lorenzon A, Costa A, Martucci M
    Placenta, 2013-11-01;35(0):S51-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Deletion of CD74, a putative MIF receptor, in mice enhances osteoclastogenesis and decreases bone mass.
    Authors: Mun S, Won H, Hernandez P, Aguila H, Lee S
    J Bone Miner Res, 2013-04-01;28(4):948-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Macrophage migration inhibitory factor inhibition is deleterious for high-fat diet-induced cardiac dysfunction.
    Authors: Palud A, Marciniak C, Montaigne D, Marechal X, Ballot C, Hassoun S, Decoster B, Neviere R, Lancel S
    PLoS ONE, 2013-03-11;8(3):e58718.
    Species: Mouse
    Sample Types: Plasma
    Applications: Bioassay
  14. Elevated serum levels of macrophage migration inhibitory factor are associated with progressive chronic cardiomyopathy in patients with Chagas disease.
    Authors: Cutrullis R, Petray P, Schapachnik E, Sanchez R, Postan M, Gonzalez M, Martin V, Corral R
    PLoS ONE, 2013-02-22;8(2):e57181.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Macrophage migration inhibitory factor homologs of anisakis simplex suppress Th2 response in allergic airway inflammation model via CD4+CD25+Foxp3+ T cell recruitment.
    Authors: Park SK, Cho MK, Park HK, Lee KH, Lee SJ, Choi SH, Ock MS, Jeong HJ, Lee MH, Yu HS
    J. Immunol., 2009-06-01;182(11):6907-14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Expression of inflammatory mediators in the otitis media induced by Helicobacter pylori antigen in mice.
    Authors: Kariya S, Okano M, Fukushima K, Nomiya S, Kataoka Y, Nomiya R, Akagi H, Nishizaki K
    Clin. Exp. Immunol., 2008-08-22;154(1):134-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Mouse MIF Protein
By Anonymous on 04/05/2024
Application: In vitro bioactivity in cell culture
Reason for Rating: The MIF I initially ordered did not perform as expected. Support went above and beyond to listen to my concerns, and identify this version of MIF as one that has a free n-terminal pro (critical for my experiment). Sure enough it worked as expected.