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Recombinant Mouse NGFR/TNFRSF16 Fc Chimera Protein, CF

Catalog #: 1157-NR
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1157-NR-050
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse NGFR/TNFRSF16 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit beta -NGF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.5-1.5 µg/mL in the presence of 2 ng/mL of rm beta -NGF.
Source
Mouse myeloma cell line, NS0-derived mouse NGFR/TNFRSF16 protein
Mouse NGF R
(Gly20 - Asn243)
Accession # Q9Z0W1.1		 DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
Q9Z0W1.1
N-terminal Sequence
Analysis
Gly20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50.4 kDa (monomer)
SDS-PAGE
75-100 kDa, reducing conditions

Product Datasheets

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1157-NR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1157-NR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NGFR/TNFRSF16

The low affinity nerve growth factor receptor (NGF R), also named p75 neurotrophin receptor, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family and has been designated TNFRSF16. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3 and NT-4 approximately equally with low affinity. The cytoplasmic region of the receptor contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R/Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in the amniotic fluid of humans and rats (1 - 3).

References
  1. Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
  2. Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
  3. Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.
Long Name
Nerve Growth Factor Receptor
Entrez Gene IDs
4804 (Human); 18053 (Mouse); 117089 (Rat)
Alternate Names
CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16

Citations for Recombinant Mouse NGFR/TNFRSF16 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The extracellular domain of neurotrophin receptor p75 as a candidate biomarker for amyotrophic lateral sclerosis.
    Authors: Shepheard S, Chataway T, Schultz D, Rush R, Rogers M
    PLoS ONE, 2014-01-27;9(1):e87398.
    Species: Human
    Sample Types: Urine
    Applications: ELISA (Standard)
  2. Soluble beta-amyloid Precursor Protein Alpha binds to p75 neurotrophin receptor to promote neurite outgrowth.
    Authors: Hasebe N, Fujita Y, Ueno M, Yoshimura K, Fujino Y, Yamashita T
    PLoS ONE, 2013-12-16;8(12):e82321.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Functional ELISA
  3. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2010-11-21;14(1):54-61.
    Applications: Surface Plasmon Resonance

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