Recombinant Mouse NPDC-1 Fc Chimera Protein, CF Summary
Product Specifications
| Mouse NPDC-1 (Ala32-Thr190, Thr33Leu) Accession # Q64322.2 | IEGRMD | Human IgG1 Fc (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10970-NP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 1 mg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Mouse NPDC-1 Fc Chimera Protein (Catalog # 10970-NP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-62 kDa and 108-124 kDa, respectively.
Reconstitution Calculator
Background: NPDC-1
Neural proliferation and differentiation control protein 1 (NPDC1) is an O-linked glycoprotein primarily expressed in lung and neural tissues and is involved in the regulation of cellular proliferation and differentiation (1,2). Mature NPDC1 consists of an extracellular domain (ECD) with a potential HLH-like domain, a transmembrane domain and a cytoplasmic domain containing a PEST motif (rich in proline, glutamine, serine, and threonine) which targets the protein for degradation (1). The ECD of mouse NPDC1 shares 76% and 92% amino acid sequence identity with human and rat NPDC1, respectively. NPDC1 expression is developmentally regulated and persists in the adult. NPDC1 binds a variety of cyclins and regulates differentiation events in neuronal precursor cells. It is expressed when neuronal precursor cells stop to divide and begin to differentiate (3). NPDC1 can interact with the transcription factor E2F-1, which results in the inhibition of E2F-1 binding to DNA and the corresponding transcription events (4). NPDC1 has been identified as a novel ligand for PILR alpha, binding through sialylation-dependent recognition (5).
- Spencer, M.L. et al. (2004) J. Biol. Chem. 279:37069.
- Evrard, C. and P. Rouget (2005) J. Neurosci. Res. 79:747.
- Galiana, E. et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:1560.
- Sansal, I. et al. (2000) Oncogene 19:5000.
- Sun, Y. et al. (2012) J Biol Chem. 287:15837.
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