Recombinant Mouse Osteopontin/OPN Protein Summary
Product Specifications
Leu17-Asn294 (Glu99Gly), with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
441-OP
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
441-OP/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 200 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Osteopontin/OPN
Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1-3). Mouse OPN is synthesized as a 294 amino acid (aa) precursor protein with a 16 aa signal peptide and a 278 aa mature protein (3). Mature mouse OPN shares 79% and 64% aa sequence identity with rat and human OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1-4). Depending on tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45-75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury
(6-8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SLAYGLR motif and at sites closer to the C-terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up-regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).
- Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
- Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
- Miyazaki, Y. et al. (1990) J. Biol. Chem. 265:14432.
- Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
- Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
- Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
- Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
- Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
- Takafuji, V. et al. (2007) Oncogene 26:6361.
- Kiefer, F.W. et al. (2010) Diabetes 59:935.
Citations for Recombinant Mouse Osteopontin/OPN Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase
Authors: Mamand, DR;Bazaz, S;Mohammad, DK;Saher, O;Wiklander, OPB;Sadeghi, B;Hassan, M;El-Andaloussi, S;Abedi-Valugerdi, M;
International immunopharmacology
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Bone marrow monocytes sustain NK cell-poiesis during non-alcoholic steatohepatitis
Authors: Bourayou, E;Perchet, T;Meunier, S;Bouvier, H;Mailhe, MP;Melanitou, E;Cumano, A;Golub, R;
Cell reports
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Increased expression of OPN contributes to idiopathic pulmonary fibrosis and indicates a poor prognosis
Authors: Ji, J;Zheng, S;Liu, Y;Xie, T;Zhu, X;Nie, Y;Shen, Y;Han, X;
Journal of translational medicine
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
A synthetic metastatic niche reveals antitumor neutrophils drive breast cancer metastatic dormancy in the lungs
Authors: Wang, J;Ocadiz-Ruiz, R;Hall, MS;Bushnell, GG;Orbach, SM;Decker, JT;Raghani, RM;Zhang, Y;Morris, AH;Jeruss, JS;Shea, LD;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Signalling by senescent melanocytes hyperactivates hair growth
Authors: Wang, X;Ramos, R;Phan, AQ;Yamaga, K;Flesher, JL;Jiang, S;Oh, JW;Jin, S;Jahid, S;Kuan, CH;Nguyen, TK;Liang, HY;Shettigar, NU;Hou, R;Tran, KH;Nguyen, A;Vu, KN;Phung, JL;Ingal, JP;Levitt, KM;Cao, X;Liu, Y;Deng, Z;Taguchi, N;Scarfone, VM;Wang, G;Paolilli, KN;Wang, X;Guerrero-Juarez, CF;Davis, RT;Greenberg, EN;Ruiz-Vega, R;Vasudeva, P;Murad, R;Widyastuti, LHP;Lee, HL;McElwee, KJ;Gadeau, AP;Lawson, DA;Andersen, B;Mortazavi, A;Yu, Z;Nie, Q;Kunisada, T;Karin, M;Tuckermann, J;Esko, JD;Ganesan, AK;Li, J;Plikus, MV;
Nature
Species: Transgenic Mouse, Xenograft
Sample Types: In Vivo
Applications: In vivo assay -
Immune stimulation recruits a subset of pro-regenerative macrophages to the retina that promotes axonal regrowth of injured neurons
Authors: Andries, L;Kancheva, D;Masin, L;Scheyltjens, I;Van Hove, H;De Vlaminck, K;Bergmans, S;Claes, M;De Groef, L;Moons, L;Movahedi, K;
Acta neuropathologica communications
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Osteopontin promotes age-related adipose tissue remodeling through senescence-associated macrophage dysfunction
Authors: D Sawaki, Y Zhang, A Mohamadi, M Pini, Z Mezdari, L Lipskaia, S Naushad, L Lamendour, DM Altintas, M Breau, H Liang, M Halfaoui, T Delmont, M Surenaud, D Rousseau, T Yoshimitsu, F Louache, S Adnot, C Henegar, P Gual, G Czibik, G Derumeaux
JCI Insight, 2023-04-24;8(8):.
Species: Mouse
Sample Types: Whole Cells
Applications: In Vivo, Bioassay -
Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease
Authors: Y Qiu, X Shen, O Ravid, D Atrakchi, D Rand, AE Wight, HJ Kim, S Liraz-Zalt, I Cooper, M Schnaider, H Cantor
Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2218915120.
Species: Mouse
Sample Types: Antibody
Applications: ELISA Capture -
Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling
Authors: Q Bai, X Wang, H Yan, L Wen, Z Zhou, Y Ye, Y Jing, Y Niu, L Wang, Z Zhang, J Su, T Chang, G Dou, Y Wang, J Sun
Journal of personalized medicine, 2023-01-11;13(1):.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Osteopontin Exacerbates High-Fat Diet-Induced Metabolic Disorders in a Microbiome-Dependent Manner
Authors: J Chen, P Zeng, L Gong, X Zhang, Z Ling, K Bi, F Shi, K Wang, Q Zhang, J Jiang, Y Zhang, T Uede, EM El-Omar, H Diao
MBio, 2022-10-27;0(0):e0253122.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
CD248 regulates Wnt signaling in pericytes to promote angiogenesis and tumor growth in lung cancer
Authors: CL Hong, IS Yu, CH Pai, JS Chen, MS Hsieh, HL Wu, SW Lin, HP Huang
Cancer Research, 2022-10-17;0(0):.
Species: Human
Sample Types: Whole Cell
Applications: Cell Culture -
Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure
Authors: FZ Khamissi, L Ning, E Kefaloyian, H Dun, A Arthanaris, A Keller, JJ Atkinson, W Li, B Wong, S Dietmann, K Lavine, D Kreisel, A Herrlich
Science Advances, 2022-02-25;8(8):eabm5900.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Osteopontin on the Dental Implant Surface Promotes Direct Osteogenesis in Osseointegration
Authors: S Makishi, T Yamazaki, H Ohshima
International Journal of Molecular Sciences, 2022-01-18;23(3):.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Inducible T-Cell Costimulator Ligand Plays a Dual Role in Melanoma Metastasis upon Binding to Osteopontin or Inducible T-Cell Costimulator
Authors: D Raineri, G Cappellano, B Vilardo, F Maione, N Clemente, E Canciani, E Boggio, CL Gigliotti, C Monge, C Dianzani, R Boldorini, U Dianzani, A Chiocchett
Biomedicines, 2021-12-27;10(1):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in mice
Authors: S Yoshida, H Nakagami, H Hayashi, Y Ikeda, J Sun, A Tenma, H Tomioka, T Kawano, M Shimamura, R Morishita, H Rakugi
Nat Commun, 2020-05-18;11(1):2482.
Species: Mouse
Sample Types: Serum
Applications: Western Blot Control -
Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin
Authors: KM Herum, A Romaine, A Wang, AO Melleby, ME Strand, J Pacheco, B Braathen, P Dunér, T Tønnessen, IG Lunde, I Sjaastad, C Brakebusch, AD McCulloch, MF Gomez, CR Carlson, G Christense
J Am Heart Assoc, 2020-01-31;9(3):e013518.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models
Authors: PG Hitscheric, LH Xie, D Del Re, EJ Lee
Physiol Rep, 2019-07-01;7(13):e14137.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Bioluminescence imaging visualizes osteopontin-induced neurogenesis and neuroblast migration in the mouse brain after stroke
Authors: R Rogall, M Rabenstein, S Vay, A Bach, A Pikhovych, J Baermann, M Hoehn, S Couillard-, GR Fink, M Schroeter, MA Rueger
Stem Cell Res Ther, 2018-07-04;9(1):182.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity
Authors: Q Zeng, X Luo, M Han, W Liu, H Li
EBioMedicine, 2018-06-07;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Megakaryocyte and Osteoblast Interactions Modulate Bone Mass and Hematopoiesis
Authors: MB Alvarez, L Xu, P Childress, KA Maupin, SFG Mohamad, B Chitteti, E Himes, DJ Olivos Iii, YH Cheng, SJ Conway, EF Srour, MA Kacena
Stem Cells Dev., 2018-05-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro
Authors: RM Natoli, H Yu, MC Meislin, P Abbasnia, P Roper, A Vuchkovska, X Xiao, SR Stock, JJ Callaci
J Orthop Surg Res, 2018-04-27;13(1):101.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Bioassay -
Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis
Authors: G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che
Immunol. Cell Biol., 2018-02-26;0(0):.
Species: Mouse
Sample Types:
Applications: In Vivo -
Genetic modifiers of muscular dystrophy act on sarcolemmal resealing and recovery from injury
Authors: M Quattrocel, J Capote, JC Ohiri, JL Warner, AH Vo, JU Earley, M Hadhazy, AR Demonbreun, MJ Spencer, EM McNally
PLoS Genet., 2017-10-24;13(10):e1007070.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Osteopontin activates mesenchymal stem cells to repair skin wound
Authors: W Wang, P Li, W Li, J Jiang, Y Cui, S Li, Z Wang
PLoS ONE, 2017-09-28;12(9):e0185346.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Osteopontin facilitates West Nile virus neuroinvasion via neutrophil Trojan horse transport
Authors: AM Paul, D Acharya, L Duty, EA Thompson, L Le, DS Stokic, AA Leis, F Bai
Sci Rep, 2017-07-05;7(1):4722.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Intravital F�rster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli
Authors: Y Kamioka, K Takakura, K Sumiyama, M Matsuda
Cancer Sci, 2017-02-01;108(2):226-235.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway
Authors: CH Chen, YM Sue, CY Cheng, YC Chen, CT Liu, YH Hsu, PA Hwang, NJ Huang, TH Chen
Sci Rep, 2017-01-18;7(0):40183.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Early gene activation initiates neuroinflammation prior to VSV neuroinvasion: Impact on antiviral responses and sleep
Authors: Richard P Ciavarra
J. Neuroimmunol, 2016-12-29;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Nanoparticle Tracking Analysis for the Enumeration and Characterization of Mineralo-Organic Nanoparticles in Feline Urine
PLoS ONE, 2016-12-22;11(12):e0166045.
Species: Feline
Sample Types: Urine
Applications: Bioassay -
Osteopontin in Spontaneous Germinal Centers Inhibits Apoptotic Cell Engulfment and Promotes Anti-Nuclear Antibody Production in Lupus-Prone Mice
J Immunol, 2016-08-17;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9
Authors: Yuta Morisaki
Sci Rep, 2016-06-06;6(0):27354.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival.
Authors: Maruyama K, Fukasaka M, Uematsu S, Takeuchi O, Kondo T, Saitoh T, Martino M, Akira S
J Biol Chem, 2015-02-17;290(15):9377-86.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Osteopontin is expressed in the mouse uterus during early pregnancy and promotes mouse blastocyst attachment and invasion in vitro.
Authors: Qi, Qian-Ron, Xie, Qing-Zhe, Liu, Xue-Li, Zhou, Yun
PLoS ONE, 2014-08-18;9(8):e104955.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Osteopontin expression by CD103- dendritic cells drives intestinal inflammation.
Authors: Kourepini E, Aggelakopoulou M, Alissafi T, Paschalidis N, Simoes D, Panoutsakopoulou V
Proc Natl Acad Sci U S A, 2014-02-18;111(9):E856-65.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
An osteopontin-integrin interaction plays a critical role in directing adipogenesis and osteogenesis by mesenchymal stem cells.
Authors: Chen Q, Shou P, Zhang L, Xu C, Zheng C, Han Y, Li W, Huang Y, Zhang X, Shao C, Roberts A, Rabson A, Ren G, Zhang Y, Wang Y, Denhardt D, Shi Y
Stem Cells, 2014-02-01;32(2):327-37.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26.
Species: Human
Sample Types: Antibody
Applications: Surface Plasmon Resonance -
Osteopontin-Stimulated Expression of Matrix Metalloproteinase-9 Causes Cardiomyopathy in the mdx Model of Duchenne Muscular Dystrophy.
Authors: Dahiya S, Givvimani S, Bhatnagar S
J. Immunol., 2011-08-01;187(5):2723-31.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR
FASEB J., 2009-08-31;24(1):70-80.
Species: Mouse
Sample Types: In Vivo, N/A
Applications: In Vivo, Western Blot -
In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression.
Authors: Marcondes MC, Poling M, Watry DD, Hall D, Fox HS
Cell. Immunol., 2008-08-03;254(1):56-62.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets.
Authors: Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V
Nat. Med., 2007-04-15;13(5):570-8.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.
Authors: Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C
Arthritis Rheum., 2004-08-01;50(8):2685-94.
Applications: ELISA (Standard) -
Angiotensin II-accelerated atherosclerosis and aneurysm formation is attenuated in osteopontin-deficient mice.
Authors: Bruemmer D, Collins AR, Noh G, Wang W, Territo M, Arias-Magallona S, Fishbein MC, Blaschke F, Kintscher U, Graf K, Law RE, Hsueh WA
J. Clin. Invest., 2003-11-01;112(9):1318-31.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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