Recombinant Mouse Siglec-E Fc Chimera Protein, CF Summary
Product Specifications
| Mouse Siglec-E (Met1-Phe355) Accession # AAH23280 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5806-SL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Mouse Siglec-E Fc Chimera (Catalog # 5806-SL) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Galectin-3 (HEK293-expressed) Protein (Catalog # 9039-GAB) binds with an ED50 of 80-800 ng/mL.
Reconstitution Calculator
Background: Siglec-E
Siglecs are sialic acid specific I‑type lectins that are characterized by an extracellular domain (ECD) with an N‑terminal Ig‑like V‑set domain followed by varying numbers of Ig‑like C2‑set domains (1, 2). Mouse Siglec‑E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 ‑ 85 kDa member of the CD33‑related subfamily of Siglecs. It consists of a 335 amino acid (aa) ECD with one Ig‑like V‑set domain and two Ig‑like C2‑set domains, a 21 aa transmembrane segment, and a 93 aa cytoplasmic domain that contains two immunoreceptor tyrosine‑based inhibitory motifs (ITIM) (3, 4). Rodent and primate Siglec gene families have significantly diverged, and Siglec‑9 is the most likely human ortholog of mouse Siglec‑E (1). Within the ECD, mouse Siglec‑E shares 56% and 80% aa sequence identity with human Siglec‑9 and rat Siglec‑E, respectively. Siglec‑E is expressed as a heavily N‑glycosylated disulfide‑linked homodimer and shows binding preference for disialic acids in the alpha 2‑8 linkage (3, 5). It is expressed on the surface of several hematopoietic cell types including neutrophils, NK cells, monocytes, peritoneal macrophages and B1 cells, and splenic myeloid dendritic cells and marginal zone B cells (5). Tyrosine phosphorylation of the cytoplasmic ITIMs mediates the association of Siglec‑E with the phosphatases SHP‑1 and SHP‑2 (3, 4). Siglec‑E is up‑regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists (6). Siglec‑E signaling negatively regulates the LPS‑induced production of TNF‑ alpha and IL‑6 by macrophages (4, 6). Its up‑regulation in macrophages parallels the development of endotoxin tolerance (6). Siglec‑E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell IL‑12 p40 production (7).
- Varki, A. and T. Angata (2006) Glycobiology 16:1R.
- Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
- Yu, Z. et al. (2001) Biochem. J. 353:483.
- Ulyanova, T. et al. (2001) J. Biol. Chem. 276:14451.
- Zhang, J.Q. et al. (2004) Eur. J. Immunol. 34:1175.
- Boyd, C.R. et al. (2009) J. Immunol. 183:7703.
- Erdmann, H. et al. (2009) Cell. Microbiol. 11:1600.
Citations for Recombinant Mouse Siglec-E Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Innate extracellular Hsp70 inflammatory properties are mediated by the interaction of Siglec-E and LOX-1 receptors
Authors: Borges, TJ;Lima, K;Murshid, A;Lape, IT;Zhao, Y;Rigo, MM;Lang, BJ;Siddiqui, SS;Hui, E;Riella, LV;Bonorino, C;Calderwood, SK;
bioRxiv : the preprint server for biology
Species: N/A
Sample Types: Protein
Applications: ELISA Capture -
Targeting stromal cell sialylation reverses T cell-mediated immunosuppression in the tumor microenvironment
Authors: Egan, H;Treacy, O;Lynch, K;Leonard, NA;O'Malley, G;Reidy, E;O'Neill, A;Corry, SM;De Veirman, K;Vanderkerken, K;Egan, LJ;Ritter, T;Hogan, AM;Redmond, K;Peng, L;Che, J;Gatlin, W;Jayaraman, P;Sheehan, M;Canney, A;Hynes, SO;Kerr, EM;Dunne, PD;O'Dwyer, ME;Ryan, AE;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CD22 blockade restores homeostatic microglial phagocytosis in ageing brains
Authors: JV Pluvinage, MS Haney, BAH Smith, J Sun, T Iram, L Bonanno, L Li, DP Lee, DW Morgens, AC Yang, SR Shuken, D Gate, M Scott, P Khatri, J Luo, CR Bertozzi, MC Bassik, T Wyss-Coray
Nature, 2019-04-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Histone Deacetylase 3 Is Required for T Cell Maturation.
Authors: Hsu F, Belmonte P, Constans M, Chen M, McWilliams D, Hiebert S, Shapiro V
J Immunol, 2015-07-10;195(4):1578-90.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
Elife, 2014-09-03;3(0):e04066.
Species: Mouse
Sample Types: Cell Lysates
Applications: Bioassay
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