Recombinant Mouse Siglec-G Fc Chimera Protein, CF Summary
Product Specifications
Recombinant Mouse Siglec-G Fc Chimera (Catalog # 10103-SL) binds to biotinylated 6'-Sialylactose-PAA.
| Mouse Siglec-G (Met19-Lys543) Accession # Q80ZE3.1 | IEGRMDP | Mouse IgG2a (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10103-SL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
In a functional ELISA. Recombinant Mouse Siglec-G Fc Chimera (Catalog # 10103-SL) binds to biotinylated 6'-Sialylactose-PAA.
View Larger
2 μg/lane of Recombinant Mouse Siglec-G Fc Chimera (Catalog # 10103-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 102-114 kDa and 205- 230 kDa, respectively.
Reconstitution Calculator
Background: Siglec-G
Siglecs are type I transmembrane proteins that belong to the immunoglobulin (Ig) superfamily and function as mammalian lectins (1). They are characterized by an extracellular domain consisting of various numbers of Ig domains with a conserved N-terminal V-set Ig ligand-binding domain. This binds species-specific sialic acid motifs on protein and lipid scaffolds to regulate intracellular signaling pathways (2). The cytoplasmic tail has signaling motifs, in most cases immunoreceptor tyrosine-based inhibitory motif (ITIM) (3). Siglec-G is a member of the CD33-related Siglec family in the mouse. It is the apparent ortholog of human Siglec-10 (4). It is expressed by mature B cells, but significant levels of transcript were also detected in DC, myeloid cells, and to a lesser extent, T cells (5). Mature Siglec-G consists of a 526 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 124 aa cytoplasmic domain. Within the ECD, mouse Siglec-G shares 63% and 86% aa sequence identity with the human and rat ortholog Siglec-10, respectively. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (6, 7). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (6). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (8, 9).
- Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
- Poe, J.C. and T.F. Tedder (2012) Trends Immunol. 33:413.
- Meyer, S.J. et al. (2018) Front. Immunol. 9:2820.
- Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
- Chen, G-Y, et al. (2014) Glycobiology 24:800.
- Li, N. et al. (2001) J. Biol. Chem. 276:28106
- Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
- Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
- Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
Citation for Recombinant Mouse Siglec-G Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
An in-situ peptide-antibody self-assembly to block CD47 and CD24 signaling enhances macrophage-mediated phagocytosis and anti-tumor immune responses
Authors: Zhang, W;Zeng, Y;Xiao, Q;Wu, Y;Liu, J;Wang, H;Luo, Y;Zhan, J;Liao, N;Cai, Y;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse Siglec-G Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse Siglec-G Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Mouse Siglec-G Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
