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Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF

Catalog #: 587-RIB
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
587-RIB-050
Recombinant Mouse TGF‑ beta RI/ALK‑5 Fc Chimera Protein Binding Activity.
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Product Details
Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Endoglin/CD105 Fc Chimera Protein (Catalog # 1320-EN) is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Mouse TGF‑ beta RI/ALK‑5 Fc Chimera (Catalog # 587-RIB) binds with an ED50 of 2.00-15.0 µg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse TGF-beta RI/ALK-5 protein
Mouse TGF-beta RI
(Ala21-Glu121) & (Thr22-Glu121)
Accession # BAA05023.1		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
BAA05023.1
N-terminal Sequence
Analysis
Ala21 & Thr22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
37.5 kDa (monomer)
SDS-PAGE
40-50 kDa, under reducing conditions.

Product Datasheets

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587-RIB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

587-RIB

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity View Larger

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Endoglin/CD105 Fc Chimera Protein (1320-EN) is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Mouse TGF‑ beta RI/ALK‑5 Fc Chimera Protein (Catalog # 587-RIB) binds with an ED50 of 2.00-15.0 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein (Catalog # 587-RIB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-50 kDa and 80-100 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RI/ALK-5

TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase (1, 2). It contains a cysteine-rich extracellular domain (ECD), a transmembrane helix, and a C-terminal cytoplasmic kinase domain (3). Within the cytoplasmic domain there is also a short, conserved regulatory sequence known as the GS region that is N-terminal to the kinase domain (1). Within the ECD, mouse TGF-beta RI shares 98% and 90% amino acid sequence identity with rat and human TGF-beta RI, respectively. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII (1). Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3 (2, 4-6). These phosphorylated Smads form heteromeric complexes with Smad4, translocate to the nucleus, and regulate target gene transcription (2, 4-6). TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells (7). Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development (8). Mutations in TGF-beta RI have been identified in pancreatic, colorectal, ovarian, and head and neck cancers (9).

References
  1. Wrana, J.L. et al. (1994) Nature 370:341.
  2. Massagué, J. (2012) Nat. Rev. Mol. Cell Biol. 13:616.
  3. Huse, M. et al. (1999) Cell 96:425.
  4. Marcías-Silva, M. et al. (1996) Cell 87:1215.
  5. Zhang, Y. et al. (1996) Nature 383:168.
  6. Huse, M. et al. (2001) Mol. Cell 8:671.
  7. Larsson, J. et al. (2001) EMBO J. 20:1663.
  8. James, J.M. et al. (2013) Development 140:3903.
  9. Sheen, Y.Y. et al. (2013) Biomol. Ther. (Seoul) 21:323.
Long Name
Transforming Growth Factor beta Receptor I
Entrez Gene IDs
7046 (Human); 21812 (Mouse); 29591 (Rat)
Alternate Names
AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I

Citations for Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011-07-07;286(34):30034-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  2. ALK1 signaling regulates early postnatal lymphatic vessel development.
    Authors: Niessen K, Zhang G, Ridgway JB, Chen H, Yan M
    Blood, 2009-11-10;115(8):1654-61.
    Applications: ELISA (Standard)

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