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Recombinant Mouse TNF-alpha (aa 84-235) Protein

Catalog #: 410-TRNC
5 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
410-TRNC-010/CF
410-TRNC-050/CF

With Carrier

Catalog # Availability Size / Price Qty
410-TRNC-010
410-TRNC-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (5)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TNF-alpha (aa 84-235) Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.8-4 pg/mL.
Source
E. coli-derived mouse TNF-alpha protein
Ser84-Leu235
Accession #
P06804
N-terminal Sequence
Analysis
Ser84
Predicted Molecular Mass
 17 kDa (monomer)

Product Datasheets

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410-TRNC (with carrier)

Product Datasheet
COA
SDS

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410-TRNC/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

410-TRNC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

410-TRNC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Mouse TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, rat, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).

References
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Fransen, L. et al. (1985) Nucleic Acids Res. 13:4417.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.
Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Citations for Recombinant Mouse TNF-alpha (aa 84-235) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Type I interferon-driven susceptibility to Mycobacterium tuberculosis is mediated by IL-1Ra
    Authors: DX Ji, LH Yamashiro, KJ Chen, N Mukaida, I Kramnik, KH Darwin, RE Vance
    Nat Microbiol, 2019-10-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Enhanced insulin receptor, but not PI3K, signalling protects podocytes from ER stress
    Authors: KL Garner, VMS Betin, V Pinto, M Graham, E Abgueguen, M Barnes, DC Bedford, CA McArdle, RJM Coward
    Sci Rep, 2018-03-02;8(1):3902.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The IVVY Motif and Tumor Necrosis Factor Receptor-associated Factor (TRAF) Sites in the Cytoplasmic Domain of the Receptor Activator of Nuclear Factor kappaB (RANK) Cooperate to Induce Osteoclastogenesis.
    Authors: Jules J, Wang S, Shi Z, Liu J, Wei S, Feng X
    J Biol Chem, 2015-08-14;290(39):23738-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Receptor activator of NF-kappaB (RANK) cytoplasmic IVVY535-538 motif plays an essential role in tumor necrosis factor-alpha (TNF)-mediated osteoclastogenesis.
    Authors: Jules J, Shi Z, Liu J, Xu D, Wang S, Feng X
    J. Biol. Chem., 2010-09-24;285(48):37427-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Functional identification of three receptor activator of NF-kappa B cytoplasmic motifs mediating osteoclast differentiation and function.
    Authors: Liu W, Xu D, Yang H, Xu H, Shi Z, Cao X, Takeshita S, Liu J, Teale M, Feng X
    J. Biol. Chem., 2004-10-12;279(52):54759-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the difference between Recombinant Mouse TNF-alpha (aa 80-235) Protein (Catalog # 410-MT) & Recombinant Mouse TNF-alpha (aa 84-235) Protein (Catalog # 410-TRNC)?

    • The mature Recombinant Mouse TNF-alpha (aa 80-235) Protein (Catalog # 410-MT) begins at amino acid 80, whereas our truncated Recombinant Mouse TNF-alpha (aa 84-235) Protein (Catalog # 410-TRNC) begins at amino acid 84. The truncated protein (Catalog # 410-TRNC) has a higher degree of bioactivity in our in-house bioassay.

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Reviews for Recombinant Mouse TNF-alpha (aa 84-235) Protein

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Recombinant Mouse TNF-alpha (aa 84-235) Protein
By Maryam Afzali on 01/13/2016
Application: In vitro bioactivity in cell culture