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Recombinant Mouse VSIG3 Fc Chimera Protein, CF

Catalog #: 9367-VS Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9367-VS-050
Recombinant Mouse VSIG3 Fc Chimera Protein Bioactivity
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Recombinant Mouse VSIG3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-5 μg/mL
Source
Mouse myeloma cell line, NS0-derived mouse VSIG3 protein
Mouse VSIG3
(Leu23-Gly245)
Accession # P0C673		IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
P0C673
N-terminal Sequence
Analysis
Leu23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa
SDS-PAGE
60-68 kDa, reducing conditions

Product Datasheets

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9367-VS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9367-VS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse VSIG3 Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse VSIG3 Fc Chimera (Catalog # 9367-VS) inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC. The ED50 for this effect is 1-5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG3

VSIG3 (V-­set and immunoglobulin domain containing 3) also known as IgSF11 (Immunoglobulin superfamily member 11), is an approximately 50 kDa type I transmembrane adhesion protein of the Ig superfamily. Mature mouse VSIG3 consists of a 218 amino acid (aa) extracellular domain (ECD) containing one V-type Ig-like domain and one Ig-like C2-type domain, a 21 aa transmembrane domain, and a 167 aa cytoplasmic domain. Within the ECD, mouse VSIG3 shares 95% and 98% aa identity with human and rat VSIG3, respectively. VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (1). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (1-3). VSIG3 also localizes to neuronal postsynaptic densities where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (4). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (5). VSIG-3 expression is elevated in colorectal cancers and hepatocellular carcinomas as well as
intestinal-type gastric cancers. Suppression of VSIG-3 by siRNA retarded the growth of gastric cancer cells, suggesting that VSIG-3 is a good candidate for gastric cancer immunotherapy (6).

References
  1. Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
  2. Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
  3. Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
  4. Jang, S. et al. (2016) Nat. Neurosci. 19:84.
  5. Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.
  6. Watanabe T, et al. (2005) Cancer Sci. 96:498.
Long Name
V-Set and Immunoglobulin Domain Containing 3
Entrez Gene IDs
152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)
Alternate Names
Brain and testis-specific immunoglobulin superfamily protein; BTIGSF; BT-IgSF; cancer/testis antigen 119; CT119; CXADR like 1; CXADRL1; IGSF11; Igsf13; immunoglobulin superfamily member 11; immunoglobulin superfamily, member 11; MGC35227; V-set and immunoglobulin domain containing 3; V-set and immunoglobulin domain-containing protein 3; VSIG3; VSIG3brain and testis-specific immunoglobin superfamily protein

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