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Recombinant Rat E-Cadherin Fc Chimera Protein, CF

Catalog #: 8144-EC
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8144-EC-050
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat E-Cadherin Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of P19 mouse embryonal carcinoma cells. The ED50 for this effect is 0.25-1.25 μg/mL
Source
Mouse myeloma cell line, NS0-derived rat E-Cadherin protein
Rat E-Cadherin
(Gln 24-Ser704)
Accession # Q9R0T4		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
Q9R0T4
N-terminal Sequence
Analysis
Asp159 & No results obtained: Gln24 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
103 kDa (pro monomer) & 87 kDa (mature monomer)
SDS-PAGE
100-125 kDa, reducing conditions

Product Datasheets

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8144-EC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8144-EC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Rat E-Cadherin shares 92% and 81% amino acid sequence identity with the mouse and human proteins, respectively (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGF R‑dependent survival and proliferation (6).

References
  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Nagafuchi, A. et al. (1987) Nature 329:341.
  3. Guillot, C. and T. Lecuit (2013) Science 340:1185.
  4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
  5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
  6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

Citations for Recombinant Rat E-Cadherin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Local contractions regulate E-cadherin rigidity sensing
    Authors: YA Yang, E Nguyen, GHN Sankara Na, M Heuzé, C Fu, H Yu, RM Mège, B Ladoux, MP Sheetz
    Science Advances, 2022-01-28;8(4):eabk0387.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. rMCP-2, the Major Rat Mucosal Mast Cell Protease, an Analysis of Its Extended Cleavage Specificity and Its Potential Role in Regulating Intestinal Permeability by the Cleavage of Cell Adhesion and Junction Proteins.
    Authors: Fu Z, Thorpe M, Hellman L
    PLoS ONE, 2015-06-26;10(6):e0131720.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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