Recombinant Rat Osteopontin (OPN) Protein, CF Summary
Product Specifications
When 1 x 105 cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.2‑1 μg/mL.
Met1-Asn317, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6359-OP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Osteopontin/OPN
Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1 ‑ 3). Rat OPN is synthesized as a 317 amino acid (aa) precursor protein with a 16 aa signal peptide and a 301 aa mature protein (3). Mature rat OPN shares 79%, 62% and 50% aa sequence identity with mouse, human and bovine OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1 ‑ 4). Depending on tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45 ‑ 75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury (6 ‑ 8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SLAYGLR motif and at sites closer to the C‑terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up‑regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).
- Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
- Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
- Oldberg, A. et al. (1986) Proc. Natl. Acad. Sci. USA 83:8819.
- Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
- Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
- Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
- Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
- Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
- Takafuji, V. et al. (2007) Oncogene 26:6361.
- Kiefer, F.W. et al. (2010) Diabetes 59:935.
Citations for Recombinant Rat Osteopontin (OPN) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Single nucleus RNA sequencing reveals glial cell type-specific responses to ischemic stroke
Authors: Bormann, D;Knoflach, M;Poreba, E;Riedl, CJ;Testa, G;Orset, C;Levilly, A;Cottereau, A;Jauk, P;Hametner, S;Golabi, B;Copic, D;Klas, K;Direder, M;Kühtreiber, H;Salek, M;Zur Nedden, S;Baier-Bitterlich, G;Kiechl, S;Haider, C;Endmayr, V;Höftberger, R;Ankersmit, HJ;Mildner, M;
bioRxiv : the preprint server for biology
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
SPP1 promotes Schwann cell proliferation and survival through PKCalpha by binding with CD44 and alphavbeta3 after peripheral nerve injury
Authors: JB Wang, Z Zhang, JN Li, T Yang, S Du, RJ Cao, SS Cui
Cell Biosci, 2020-08-20;10(0):98.
Species: Human, Rat
Sample Types: Whole Cells
Applications: Bioassay -
Chromatin organization regulated by EZH2-mediated H3K27me3 is required for OPN-induced migration of bone marrow-derived mesenchymal stem cells
Authors: L Liu, Q Luo, J Sun, Y Ju, Y Morita, G Song
Int. J. Biochem. Cell Biol., 2018-01-11;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
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