Recombinant Zebrafish VEGF 165 Protein

Catalog #: 1247-ZV
2 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
1247-ZV-010/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
1247-ZV-010

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (2)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Zebrafish VEGF 165 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 20-60 ng/mL.

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived zebrafish VEGF protein
Ala24-Arg188

Accession #

O73682.1

N-terminal Sequence
Analysis

Ala24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

19.2 kDa (monomer)

SDS-PAGE

22 kDa, reducing conditions

Product Datasheets

1247-ZV (with carrier)

Product Datasheet

COA

SDS

1247-ZV/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1247-ZV

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1247-ZV/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: VEGF

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternate splice isoforms of VEGF-A, ranging from 206 amino acids (aa) to 121 aa in length, are known. In zebrafish, two VEGF isoforms, a 165 aa and a 121 aa isoform, have been reported. Mature zebrafish VEGF₁₆₅ shares 64%, 62% and 62% aa sequence identity with frog, human, and mouse VEGF₁₆₅, respectively. There are two tyrosine kinase receptors for VEGF reported in mammals termed VEGF R1 and VEGF R2/FLK-1. One receptor has been identified in zebrafish(FLK-1), and this may actually represent the orthologue to the early common ancestor for mammalian VEGF R1 and R2. All receptors are type I transmembrane proteins that show seven immunoglobulin-like domains extracellularly and a split kinase domain intracellularly. In addition to the tyrosine kinase receptors, neuropilin-1 (NRP-1) has been reported to be a coreceptor for VEGF binding. It is proposed that the presence of NRP-1 lowers the concentration of VEGF necessary for activation of VEGF R2. NRP-1 has been reported in both zebrafish and human. VEGF regulates multiple biological functions in endothelial cells, including cell proliferation, migration and survival. These functions of VEGF are mediated partly through the induction of nitric oxide and prostacyclin, as well as upregulation of metalloproteinases. Together with other vascular-specific growth factors such as the Angiopoietins, VEGF have separate but complementary roles in angiogenesis and vasculogenesis (1‑7).

References

Laing, D. et al. (1998) Biochem. Biophys. Acta 1397:14.
Laing, D. and R. Ge (1998) GenBank Accession # AAC14713.
Laio, W. et al. (1997) Development 124:381.
Lee, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10470.
Thurston, G. (2002) J. Anat. 200:575.
Zachary, I. and G. Gliki (2001) Cardiovasc. Res. 49:568.
Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.

Long Name

Vascular Endothelial Growth Factor

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Related Research Areas

Citations for Recombinant Zebrafish VEGF 165 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Overexpression of preeclampsia induced microRNA-26a-5p leads to proteinuria in zebrafish
Authors: J Müller-Dei, P Schröder, L Beverly-St, R Hiss, J Fiedler, J Nyström, T Thum, H Haller, M Schiffer
Sci Rep, 2018-02-26;8(1):3621.
Species: Zebrafish
Sample Types: In Vivo
Applications: In Vivo
Expression and mapping of duplicate neuropilin-1 and neuropilin-2 genes in developing zebrafish.
Authors: Bovenkamp DE, Goishi K, Bahary N, Davidson AJ, Zhou Y, Becker T, Becker CG, Zon LI, Klagsbrun M
Gene Expr. Patterns, 2004-07-01;4(4):361-70.
Species: Zebrafish
Sample Types: Recombinant Protein
Applications: Binding Assay