Serum-free HEK293 Transfection Media

Media for the serum-free transfection of HEK293 cells.

Why use serum-free media for the transfection of HEK293 cells?

Human Embryonic Kidney (HEK)293 cells are a commonly used mammalian cell line for the expression of recombinant proteins and antibodies. Transfecting and expanding HEK293 cells under serum-free conditions allows for the researcher to have a more controlled and defined system.

Serum-free HEK Transfection Media is formulated to support transfection of HEK293 cells for recombinant protein and antibody expression in suspension culture under serum-free conditions. The media has been tested for its ability to support transfection of high-density suspension cultures of HEK293EBNA cells adapted from adherent culture platforms.

This kit contains the following reagents to transfect HEK293 cells in suspension culture:

• Serum-free HEK Transfection Media (500 mL)

Stability and Storage

This media should be stored at 2 – 8 ^°C. Protect from light. Use within the expiration date listed on the bottle. For optimal culture performance, bottles should be used within 1 month after opening to prevent loss of CO₂ and elevated pH.

Precaution

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, these reagents should be handled as if capable of transmitting infection. When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• This reagent should not be used beyond the expiration date indicated on the label.

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Serum-free HEK Transfection Media Supports Higher Antibody Expression in HEK293 Cells. HEK293 cells were expanded to a concentration of 2-3 x 106 cell/mL in Serum-free HEK Cell Culture Media (R&D Systems®, Catalog # CCM023). Cells were then spun down and resuspended at 5 x 106 cells/mL in Serum-free HEK Transfection Media or one of two other commercially available medias (Competitor 1 and Competitor 2). Each cell suspension was then transfected with a plasmid encoding one of two different antibodies (Antibody 1 - green bars; Antibody 2 - blue bars). After transfection, each culture was diluted with an equal volume of cell culture media, either competitor's or Serum-free HEK Cell Culture Media (R&D Systems®, Catalog # CCM023). The cultures were maintained for an additional 5-7 days prior to assessing antibody expression by ELISA.