Simple Plex Human Clusterin Cartridge

Catalog #: SPCKB-PS-001512 Datasheet / COA / SDS

Simple Plex Human Clusterin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001512
SPCKA-PS-001512
SPCKC-PS-001512

Control Products Available

898981: Simple Plex Control for Human Clusterin

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Clusterin Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (3 nL), EDTA Plasma (3 nL), Heparin Plasma (3 nL), Urine (500 nL)

Sensitivity

6.14 pg/mL

Assay Range

40.5 - 386,400 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human Clusterin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Clusterin in serum, plasma (EDTA/Heparin), and urine

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	44	43
Mean (pg/mL)	2303	103870	2317	111162
Standard Deviation	101	4562	260	8163
CV%	4.4	4.4	11.2	7.3

Recovery

Recovery was not necessary as Clusterin shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Clusterin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human Clusterin Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Clusterin

Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40,40, is a secreted multifunctional protein that binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis. Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development. It is secreted as a disulfide-linked heterodimer of alpha and beta chains and circulates as a component of high density lipoprotein (HDL) particles. Clusterin exerts tissue protective functions in atherosclerosis and Alzheimer’s disease by binding and neutralizing non-oxidatively modified LDL reduces cytotoxicity and beta-amyloid fibrils, respectively. Increased circulating levels of Clusterin enhance tumor aggressiveness by inhibiting apoptosis and by promoting epithelial to mesenchymal transition.

Entrez Gene IDs:

1191 (Human); 12759 (Mouse)

Alternate Names:

40; 40, sulfated glycoprotein 2; Aging-associated gene 4 protein; aging-associated protein 4; APOJ; apo-J; Apolipoprotein J; CLI; CLIclusterin (complement lysis inhibitor, SP-40; CLU; Clusterin; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40; Ku70-binding protein 1; KUB1SGP2; MGC24903; NA1/NA2; SGP-2; SP-40; sulfated glycoprotein 2; Testosterone-repressed prostate message 2; testosterone-repressed prostate message 2, apolipoprotein J); TRPM-2; TRPM-2TRPM2