Simple Plex Human MICA Cartridge

Catalog #: SPCKB-PS-000197 Datasheet / COA / SDS

Simple Plex Human MICA assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000197
SPCKA-PS-000197
SPCKC-PS-000197

Control Products Available

898141: Simple Plex Control for Human MICA

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All MICA Simple Plex Assays

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human MICA Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.31 pg/mL

Assay Range

5.1 - 7,800 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MICA.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human MHC class I Chain-related gene A (MICA) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	105	101-109
EDTA Plasma (n=4)	91	73-102
Heparin Plasma (n=4)	96	87-102
Serum (n=4)	99	77-111

Linearity

Samples containing and/or spiked with high concentrations of human MICA were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human MICA Simple Plex Assay Standard Curve

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Human MICA Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MICA

MHC class I chain-related genes MICA and MICB are transmembrane glycoproteins that function as ligands for human NKG2D. The two proteins are highly related, sharing 85% amino acid identity, but are also polymorphic. Recognition of MICA or MICB by NKG2D results in the activation of NK cell cytolytic activity and/or cytokine production. MICA/B are minimally expressed on normal cells, but are frequently expressed on or shed from epithelial tumors and can be induced by bacterial and viral infections. MICA and MICB recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

Long Name:

MHC Class I-related Protein A

Entrez Gene IDs:

100507436 (Human)

Alternate Names:

FLJ60820; MGC111087; MICA; PERB11.1