Simple Plex Human u-Plasminogen Activator Cartridge

Catalog #: SPCKB-PS-000538 Datasheet / COA / SDS

Simple Plex Human u-Plasminogen Activator/uPA/Urokinase assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000538
SPCKA-PS-000538
SPCKC-PS-000538

Control Products Available

898122: Simple Plex Control for Human u-Plasminogen Activator (uPA)

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All u-Plasminogen Activator (uPA)/Urokinase Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human u-Plasminogen Activator Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (10 uL)

Sensitivity

1.33 pg/mL

Assay Range

3.9 - 15,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human uPA.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Neurogranin in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and urine.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	22	21
Mean (pg/mL)	70.7	3769	72.3	3684
Standard Deviation	3.4	257	10.2	413
CV%	4.8	6.8	14.1	11.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	99	97-100

Linearity

Samples containing and/or spiked with high concentrations of human uPA were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human uPA Simple Plex Assay Standard Curve

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Human uPA Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: u-Plasminogen Activator (uPA)/Urokinase

u-Plasminogen Activator (uPA) is a serine protease that converts plasminogen to plasmin, with roles in a variety of normal and pathological processes that include cell migration and tissue destruction. uPA is a potent marker of invasion and metastasis in a variety of human cancers including breast, stomach, colon, bladder, ovarian, brain, and endometrium.

Long Name:

Urokinase-type Plasminogen Activator

Entrez Gene IDs:

5328 (Human); 18792 (Mouse); 102135886 (Cynomolgus Monkey)

Alternate Names:

ATF; EC 3.4.21; EC 3.4.21.73; plasminogen activator, urokinase; PLAU; uPA; u-PA; uPlasminogen Activator; u-Plasminogen Activator; urinary; Urokinase; urokinase-type plasminogen activator