Simple Plex Human VEGF Cartridge

Name: Simple Plex Human VEGF Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000330
Rating: 5 (1 reviews)

Catalog #: SPCKB-PS-000330 1 Review Datasheet / COA / SDS

Simple Plex Human VEGF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. CSF samples are validated in SD13 only, it is not recommended to use SD06

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000330
SPCKA-PS-000330
SPCKC-PS-000330

Control Products Available

894978: Simple Plex Control for Human VEGF

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human VEGF Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)

Sensitivity

1.75 pg/mL

Assay Range

3.0 - 4,562 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma), 3.7 - 5,570 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant human (rh) VEGF₁₁₁, rhVEGF₁₂₁, rhVEGF₁₄₅, rhVEGF₁₆₅, and rhVEGF₁₈₉.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Vascular Endothelial Growth Factor A (VEGF-A) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin), and cerebrospinal fluid (CSF). CSF samples are validated in SD13 only, it is not recommended to use SD06.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	8	10
Mean (pg/mL)	26.1	1126	1.33	72.3
Standard Deviation	1.33	72.3	1.9	139
CV%	5.1	6.4	6.6	10.8

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	8	10
Mean (pg/mL)	34.5	1519	33.3	1440
Standard Deviation	1.32	109	2.38	89.6
CV%	3.8	7.1	7.1	6.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cerebrospinal Fluid (n=6)	114	110-119
EDTA Plasma (n=3)	91	82-100
Heparin Plasma (n=3)	86	75-94
Serum (n=3)	95	82-106

Linearity

Samples containing and/or spiked with high concentrations of human VEGF-A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Human VEGF Ella Assay Linearity (Sample Diluent SD06)

Scientific Data

N/A

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Human VEGF Ella Assay Standard Curve (Sample Diluent 13)

N/A

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Human VEGF Ella Assay Standard Curve (Sample Diluent 06)

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

Long Name:

Vascular Endothelial Growth Factor

Entrez Gene IDs:

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names:

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor