Home / TGF-beta 1, 2, 3 / TGF-beta 1, 2, 3 APC-conjugated Antibody

TGF-beta 1, 2, 3 APC-conjugated Antibody

Catalog #: IC1835A
3 Citations Datasheet / COA / SDS

Discontinued Product

IC1835A has been discontinued.
View all TGF-beta 1, 2, 3 products.
Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry.
1 Image
Product Details
Citations (3)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

TGF-beta 1, 2, 3 APC-conjugated Antibody Summary

Specificity
Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
Source
Monoclonal Mouse IgG1 Clone # 1D11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Bovine bone-derived TGF-beta 1 and TGF-beta 2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of TGF-beta 1, 2, 3 antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 APC-conjugated Mono-clonal Antibody (Catalog # IC1835A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta 1, 2, 3

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

References
  1. Ayala A. et al. (1992) FASEB J. 6:A1604.
  2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
  3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.
Long Name
Transforming Growth Factor beta 1, 2, and 3
Alternate Names
CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for TGF-beta 1, 2, 3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Activated Chicken Gamma Delta T Cells Are Involved in Protective Immunity against Marek's Disease
    Authors: A Matsuyama-, B Shojadoost, N Boodhoo, S Raj, M Alizadeh, F Fazel, C Fletcher, J Zheng, B Gupta, MF Abdul-Care, BL Plattner, S Behboudi, S Sharif
    Viruses, 2023-01-19;15(2):.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-10 inhibits neuraminidase-activated TGF-beta and facilitates Th1 phenotype during early phase of infection.
    Authors: Dutta A, Huang C, Chen T, Lin C, Chiu C, Lin Y, Chang C, He Y
    Nat Commun, 2015-03-02;6(0):6374.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Mucosal tolerance to a combination of ApoB and HSP60 peptides controls plaque progression and stabilizes vulnerable plaque in Apob(tm2Sgy)Ldlr(tm1Her)/J mice.
    Authors: Mundkur L, Mukhopadhyay R, Samson S, Varma M, Kale D, Chen D, Shivaprasad S, Sivanandan H, Soman V, Lu X, Kakkar V
    PLoS ONE, 2013-03-11;8(3):e58364.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for TGF-beta 1, 2, 3 APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review TGF-beta 1, 2, 3 APC-conjugated Antibody and earn rewards!

Have you used TGF-beta 1, 2, 3 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review