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TGF-beta 1 Antibody

Catalog #: MAB240
88 Citations 9 Reviews Datasheet / COA / SDS
Newer Version Available: MAB1835
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Discontinued Product

MAB240 has been discontinued and is replaced by MAB1835. MAB2402.

TGF‑ beta 1 in HEK293 Human Cell Line.
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Product Details
Citations (88)
FAQs
Reviews (9)
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TGF-beta 1 Antibody Summary

Specificity
Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than
2% cross-reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 9016
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TGF-beta 1 (Catalog # 240-B)
under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 0.25 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TGF-beta 1 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

TGF‑ beta 1 in HEK293 Human Cell Line. TGF-beta 1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-TGF-beta 1 Monoclonal Antibody (Catalog # MAB240) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry TGF-beta 1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

TGF-beta 1 in Human Prostate Cancer Tissue. TGF-beta 1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti- TGF-beta 1 Monoclonal Antibody (Catalog # MAB240) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in the prostate gland. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of TGF-beta 1 antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TGF‑ beta 1 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1 Monoclonal Antibody (Catalog # MAB240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Neutralization TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1 Antibody. View Larger

TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1 Antibody. Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF-beta 1 Monoclonal Antibody (Catalog # MAB240). The ND50 is typically 0.3-1.0 µg/mL.

Western Blot Detection of Mouse TGF-beta 1 by Western Blot View Larger

Detection of Mouse TGF-beta 1 by Western Blot ERK and TGF-beta 1 levels in IBD-A33+ Li-EVs.(a) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38 cells or MC38-EVs was measured using western blot analysis. (b) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for the indicated times. Activation of ERK, JNK, p38 and ATK in MC38 cells was measured using western blot analysis. (c) MC38 cells were pretreated with DMSO, 10 μM ERK-specific inhibitor U0126 or JNK-specific inhibitor SP600125 for 30 min and then treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38-EVs was measured by western blot analysis. (d) Activation of ERK, JNK, p38 and ATK in colon tissues from healthy control or IBD mice was measured using western blot analysis. (e,f) Mice were fed with 2% DSS solution on day 0. Mice received intrarectal injection with 20 μg cholesterol-conjugated ERK or NC siRNA every other day on days 0–11. Twenty-four hours after the last injection, mice were killed. TGF-beta 1 in colon tissues and A33+ Li-EVs was measured using western blot analysis (e). The body weights of mice were measured daily (f). Ctrl group, mice that received normal drinking water. (a–e) Data are representative of three independent experiments; (f) data are presented as the mean±s.d. from one of the two independent experiments (n=5 per group). P values were generated by two-way ANOVA, followed by Newman–Keuls multiple comparison test using GraphPad Prism 5 (**P<0.01, ***P<0.001), IBD+NC siRNA versus IBD+ERK siRNA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27721471), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse TGF-beta 1 by Western Blot View Larger

Detection of Mouse TGF-beta 1 by Western Blot ERK and TGF-beta 1 levels in IBD-A33+ Li-EVs.(a) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38 cells or MC38-EVs was measured using western blot analysis. (b) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for the indicated times. Activation of ERK, JNK, p38 and ATK in MC38 cells was measured using western blot analysis. (c) MC38 cells were pretreated with DMSO, 10 μM ERK-specific inhibitor U0126 or JNK-specific inhibitor SP600125 for 30 min and then treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38-EVs was measured by western blot analysis. (d) Activation of ERK, JNK, p38 and ATK in colon tissues from healthy control or IBD mice was measured using western blot analysis. (e,f) Mice were fed with 2% DSS solution on day 0. Mice received intrarectal injection with 20 μg cholesterol-conjugated ERK or NC siRNA every other day on days 0–11. Twenty-four hours after the last injection, mice were killed. TGF-beta 1 in colon tissues and A33+ Li-EVs was measured using western blot analysis (e). The body weights of mice were measured daily (f). Ctrl group, mice that received normal drinking water. (a–e) Data are representative of three independent experiments; (f) data are presented as the mean±s.d. from one of the two independent experiments (n=5 per group). P values were generated by two-way ANOVA, followed by Newman–Keuls multiple comparison test using GraphPad Prism 5 (**P<0.01, ***P<0.001), IBD+NC siRNA versus IBD+ERK siRNA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27721471), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse TGF-beta 1 by Western Blot View Larger

Detection of Mouse TGF-beta 1 by Western Blot A33+ Li-EVs alleviate IBD through TGF-beta 1 signalling.(a) A33+ Li-EVs were isolated from healthy control or 2% DSS-induced IBD mice. TGF-beta 1, A33 and beta -Actin were detected by western blot analysis. (b) The CD4+ T-cell proliferation assay was performed as described above in the presence of 30 μg ml−1 Ctrl or IBD-A33+ Li-EVs, and the results were statistically analysed (n=9). (c–f) Mice were fed with drinking water containing 2% DSS on day 0. On days −2 and 2, the mice were intravenously treated with the indicated dose of A33+ Li-EVs. The body weights were measured daily (c). Appearance (left) and statistical analysis (right) of colonic length on days 11 (d). Histological appearance on day 11. Representative colonic sections stained with haematoxylin and eosine (H&E). In the PBS group, the right image is a magnified region of the left image (e). IL-1 beta, IL-6, TNF-alpha, IL-10, IL-22 levels and MPO activity in colon tissue were measured on day 11 (f). (g) IBD mice were treated with 100 μg A33+ Li-EVs, IBD-A33+ Li-EVs and Sp-EVs on days −2 and 2. The body weights were measured daily. (h) TGF-beta 1 in A33+ Li-EVs and Sp-EVs was detected using western blot analysis. (i) IBD was introduced into Smad3+/− mice and treated with 100 μg A33+ Li-EVs and IBD-A33+ Li-EVs on days −2 and 2. The body weights were measured daily. Ctrl group, mice received normal drinking water; PBS group, mice with drinking water containing 2% DSS and intravenously treated with PBS on days −2 and 2. (a,b,d–f) Data are representative of three independent experiments or shown as mean values±s.e.m. pooled from three independent experiments; (c,g,i) data are presented as the mean±s.d. from one of the three independent experiments (n=5 per group). P values were generated by one-way ANOVA in b,d,f, or two-way ANOVA in c,g,i, followed by Newman–Keuls multiple comparison test using GraphPad Prism 5 (*P<0.05, **P<0.01, ***P<0.001), corresponding colour indicating the relevant group versus PBS group in c, versus PBS group in d,f; IBD-A33+ Li-EVs versus A33+ Li-EVs in g. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27721471), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human TGF-beta 1 by Flow Cytometry View Larger

Detection of Human TGF-beta 1 by Flow Cytometry CD4+ Vδ1+ T-cell clones have a TH0-cytokine profile and are effector-memory cells. (A) CD4+ Vδ1+ clones produce regulatory IL-10 and TGF-beta, TH2 cytokines IL-4, -5, and -13, and to a lesser extent proinflammatory IL-17A and IL-6, and the TH1-cytokines IL-2, IFN-gamma, and TNF-alpha after stimulation with PMA/ionomycin. (B) CD4+ Vδ1+ clones were not naïve but belonged to the effector memory; they were CD45RA−, CD27−, CCR7−, and predominantly CD62L− and CD28±. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fimmu.2014.00645/abstract), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse TGF-beta 1 by Western Blot View Larger

Detection of Mouse TGF-beta 1 by Western Blot ERK and TGF-beta 1 levels in IBD-A33+ Li-EVs.(a) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38 cells or MC38-EVs was measured using western blot analysis. (b) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for the indicated times. Activation of ERK, JNK, p38 and ATK in MC38 cells was measured using western blot analysis. (c) MC38 cells were pretreated with DMSO, 10 μM ERK-specific inhibitor U0126 or JNK-specific inhibitor SP600125 for 30 min and then treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38-EVs was measured by western blot analysis. (d) Activation of ERK, JNK, p38 and ATK in colon tissues from healthy control or IBD mice was measured using western blot analysis. (e,f) Mice were fed with 2% DSS solution on day 0. Mice received intrarectal injection with 20 μg cholesterol-conjugated ERK or NC siRNA every other day on days 0–11. Twenty-four hours after the last injection, mice were killed. TGF-beta 1 in colon tissues and A33+ Li-EVs was measured using western blot analysis (e). The body weights of mice were measured daily (f). Ctrl group, mice that received normal drinking water. (a–e) Data are representative of three independent experiments; (f) data are presented as the mean±s.d. from one of the two independent experiments (n=5 per group). P values were generated by two-way ANOVA, followed by Newman–Keuls multiple comparison test using GraphPad Prism 5 (**P<0.01, ***P<0.001), IBD+NC siRNA versus IBD+ERK siRNA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27721471), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1

TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

Long Name
Transforming Growth Factor beta 1
Entrez Gene IDs
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names
CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

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Citations for TGF-beta 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

88 Citations: Showing 1 - 10
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  1. ZBTB18 restricts chromatin accessibility and prevents transcriptional adaptations that drive metastasis
    Authors: R Wang, AB Bhatt, BA Minden-Bir, OK Travis, S Tiwari, H Jia, W Rosikiewic, O Martinot, E Childs, R Loesch, G Tossou, S Jamieson, D Finkelstei, B Xu, M Labelle
    Science Advances, 2023-01-06;9(1):eabq3951.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Intermittent compressive force regulates dentin matrix protein 1 expression in human periodontal ligament stem cells
    Authors: J Manokawinc, S Chareonvit, V Trachoo, P Limraksasi, H Egusa, T Osathanon
    Journal of dental sciences, 2022-07-15;18(1):105-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Cardiac fibroblasts regulate the development of heart failure via Htra3-TGF-beta-IGFBP7 axis
    Authors: T Ko, S Nomura, S Yamada, K Fujita, T Fujita, M Satoh, C Oka, M Katoh, M Ito, M Katagiri, T Sassa, B Zhang, S Hatsuse, T Yamada, M Harada, H Toko, E Amiya, M Hatano, O Kinoshita, K Nawata, H Abe, T Ushiku, M Ono, M Ikeuchi, H Morita, H Aburatani, I Komuro
    Nature Communications, 2022-06-07;13(1):3275.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Tumor-infiltrating lymphocytes are functionally inactivated by CD90+ stromal cells and reactivated by combined Ibrutinib and Rapamycin in human pleural mesothelioma
    Authors: H Yang, S Berezowska, P Dorn, P Zens, P Chen, RW Peng, TM Marti, GJ Kocher, RA Schmid, SRR Hall
    Theranostics, 2022-01-01;12(1):167-185.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Targeting Cpt1a-Bcl-2 interaction modulates apoptosis resistance and fibrotic remodeling
    Authors: L Gu, R Surolia, JL Larson-Cas, C He, D Davis, J Kang, VB Antony, AB Carter
    Cell Death and Differentiation, 2021-08-20;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. &alphaSMA+ fibroblasts suppress Lgr5+ cancer stem cells and restrain colorectal cancer progression
    Authors: KM McAndrews, K Vázquez-Ar, C Kwak, H Sugimoto, X Zheng, B Li, ML Kirtley, VS LeBleu, R Kalluri
    Oncogene, 2021-06-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. TGF-&beta loaded exosome enhances ischemic wound healing in vitro and in vivo
    Authors: A Shi, J Li, X Qiu, M Sabbah, S Boroumand, TC Huang, C Zhao, A Terzic, A Behfar, SL Moran
    Theranostics, 2021-04-30;11(13):6616-6631.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  8. Active food ingredients production from cold pressed processing residues of Camellia oleifera and Camellia sinensis seeds for regulation of blood pressure and vascular function
    Authors: SS Chiang, LS Chen, CY Chu
    Chemosphere, 2020-12-11;267(0):129267.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Assessment of the functional efficacy of root canal treatment with high-frequency waves in rats
    Authors: S Matsui, N Yoneda, H Maezono, K Kuremoto, T Ishimoto, T Nakano, H Yumoto, S Ebisu, Y Noiri, M Hayashi
    PLoS ONE, 2020-09-29;15(9):e0239660.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles
    Authors: LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka
    Nat Commun, 2020-05-11;11(1):2331.
    Species: Human
    Sample Types: Whole Cells
    Applications: SEM
  11. Neutrophils from severe asthmatic patients induce epithelial to mesenchymal transition in healthy bronchial epithelial cells
    Authors: A Haddad, M Gaudet, M Plesa, Z Allakhverd, AK Mogas, S Audusseau, CJ Baglole, DH Eidelman, R Olivenstei, MS Ludwig, Q Hamid
    Respir. Res., 2019-10-29;20(1):234.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  12. Response gene to complement 32 expression in macrophages augments paracrine stimulation-mediated colon cancer progression
    Authors: P Zhao, B Wang, Z Zhang, W Zhang, Y Liu
    Cell Death Dis, 2019-10-10;10(10):776.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Intermittent compressive force promotes osteogenic differentiation in human periodontal ligament cells by regulating the transforming growth factor-beta pathway
    Authors: J Manokawinc, P Pavasant, C Sawangmake, N Limjeeraja, CN Limjeeraja, H Egusa, T Osathanon
    Cell Death Dis, 2019-10-07;10(10):761.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  14. Adipose-derived stem cells prevent the onset of bisphosphonate-related osteonecrosis of the jaw through transforming growth factor ?-1-mediated gingival wound healing
    Authors: X Zang, L He, L Zhao, Y He, E Xiao, Y Zhang
    Stem Cell Res Ther, 2019-06-13;10(1):169.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction
    Authors: CL Lin, YC Hsu, YT Huang, YH Shih, CJ Wang, WC Chiang, PJ Chang
    EMBO Mol Med, 2019-05-01;11(5):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Elevated circulating TGF?1 during acute liver failure activates TGF?R2 on cortical neurons and exacerbates neuroinflammation and hepatic encephalopathy in mice
    Authors: M McMillin, S Grant, G Frampton, AD Petrescu, E Williams, B Jefferson, A Thomas, A Brahmarout, S DeMorrow
    J Neuroinflammation, 2019-04-02;16(1):69.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. Cryptotanshinone Ameliorates Radiation-Induced Lung Injury in Rats
    Authors: Y Jiang, F You, J Zhu, C Zheng, R Yan, J Zeng
    Evid Based Complement Alternat Med, 2019-02-20;2019(0):1908416.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Association between FOXP3+ regulatory T-cells and occurrence of peritoneal lesions in women with ovarian endometrioma and dermoid cysts
    Authors: KN Khan, K Yamamoto, A Fujishita, A Koshiba, H Kuroboshi, S Sakabayash, S Teramukai, M Nakashima, J Kitawaki
    Reprod. Biomed. Online, 2019-01-31;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Spatio-temporal overview of neuroinflammation in an experimental mouse stroke model
    Authors: L Buscemi, M Price, P Bezzi, L Hirt
    Sci Rep, 2019-01-24;9(1):507.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge
    Authors: S Yi, J Zhai, R Niu, G Zhu, M Wang, J Liu, H Huang, Y Wang, X Jing, L Kang, W Song, Y Shi, H Tang
    Nat Commun, 2018-09-24;9(1):3879.
  21. Targeted deletion of T cell S1P receptor 1 ameliorates cardiac fibrosis in streptozotocin-induced diabetic mice
    Authors: CS Abdullah, ZQ Jin
    FASEB J., 2018-04-26;0(0):fj201800231R.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Specific Inhibitor of Smad3 (SIS3) Attenuates Fibrosis, Apoptosis, and Inflammation in Unilateral Ureteral Obstruction Kidneys by Inhibition of Transforming Growth Factor ? (TGF-?)/Smad3 Signaling
    Authors: X Ji, H Wang, Z Wu, X Zhong, M Zhu, Y Zhang, R Tan, Y Liu, J Li, L Wang
    Med. Sci. Monit., 2018-03-20;24(0):1633-1641.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  23. CD8?+CD11c+ Extracellular Vesicles in the Lungs Control Immune Homeostasis of the Respiratory Tract via TGF-?1 and IL-10
    Authors: S Wan, S Wang, L Weng, G Zhang, Z Lin, X Fei, F Zhang, F Yang, J Wang, Z Cai
    J. Immunol., 2018-01-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay, Neutralization
  24. Human amniotic epithelial cells regulate osteoblast differentiation through the secretion of TGF?1 and microRNA-34a-5p
    Authors: G Wang, F Zhao, D Yang, J Wang, L Qiu, X Pang
    Int. J. Mol. Med., 2017-11-17;41(2):791-799.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  25. Fibroblast deletion of ROCK2 attenuates cardiac hypertrophy, fibrosis, and diastolic dysfunction
    Authors: T Shimizu, N Narang, P Chen, B Yu, M Knapp, J Janardanan, J Blair, JK Liao
    JCI Insight, 2017-07-06;2(13):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Suppression of Hepatic Epithelial-to-Mesenchymal Transition by Melittin via Blocking of TGF?/Smad and MAPK-JNK Signaling Pathways
    Authors: JH Park, B Park, KK Park
    Toxins (Basel), 2017-04-13;9(4):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. FOXO1 expression in keratinocytes promotes connective tissue healing
    Authors: C Zhang, J Lim, J Liu, B Ponugoti, S Alsadun, C Tian, R Vafa, DT Graves
    Sci Rep, 2017-02-21;7(0):42834.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration
    Authors: O Miroshnych, WT Chang, JL Dragoo
    Am J Sports Med, 2016-12-27;45(4):945-953.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  29. A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood
    Nat Commun, 2016-11-22;7(0):13363.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  30. Role of urotensin�II in advanced glycation end product-induced extracellular matrix synthesis in rat proximal tubular epithelial cells
    Authors: Jiping Qi
    Int. J. Mol. Med., 2016-10-26;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  31. Platelet-Derived Ectosomes Reduce NK Cell Function
    J Immunol, 2016-07-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  32. Macrophage TGF-?1 and the Proapoptotic Extracellular Matrix Protein BIGH3 Induce Renal Cell Apoptosis in Prediabetic and Diabetic Conditions
    Authors: RJ Moritz, RG LeBaron, CF Phelix, R Rupaimoole, HS Kim, A Tsin, R Asmis
    Int J Clin Med, 2016-07-21;7(7):496-510.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  33. A novel immunomodulatory function of neutrophils on rhinovirus-activated monocytes in vitro
    Authors: Brian G Oliver
    Thorax, 2016-06-10;71(11):1039-1049.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  34. Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis.
    Authors: Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J
    Nat Commun, 2016-03-18;7(0):11012.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  35. Manual therapy as an effective treatment for fibrosis in a rat model of upper extremity overuse injury
    Authors: GM Bove, MY Harris, H Zhao, MF Barbe
    J. Neurol. Sci, 2015-12-24;361(0):168-80.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  36. Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
    Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
    J Immunol, 2015-07-31;195(5):2141-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  37. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
    Authors: Sommer A, Fabri M
    PLoS ONE, 2015-06-24;10(6):e0130395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  38. Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-beta1.
    Authors: Pahlman L, Jogi A, Gram M, Mori M, Egesten A
    BMC Pulm Med, 2015-03-07;15(0):19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.
    Authors: Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K
    Nat Commun, 2015-02-19;6(0):6329.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  40. Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
    Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
    Proc Natl Acad Sci U S A, 2015-02-17;112(9):E957-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination.
    Authors: Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z
    Eur J Immunol, 2015-02-01;45(2):442-51.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Neutralization
  42. Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-ss
    Authors: Ogawa H, Ledford J, Mukherjee S, Aono Y, Nishioka Y, Lee J, Izumi K, Hollingsworth J
    Respir Res, 2014-11-29;15(1):143.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  43. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014-05-13;111(21):E2229-36.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  44. Mesenchymal stem cells cancel azoxymethane-induced tumor initiation.
    Authors: Nasuno M, Arimura Y, Nagaishi K, Isshiki H, Onodera K, Nakagaki S, Watanabe S, Idogawa M, Yamashita K, Naishiro Y, Adachi Y, Suzuki H, Fujimiya M, Imai K, Shinomura Y
    Stem Cells, 2014-04-01;32(4):913-25.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  45. Regulation of renal fibrosis by Smad3 Thr388 phosphorylation.
    Authors: Qu X, Li X, Zheng Y, Ren Y, Puelles V, Caruana G, Nikolic-Paterson D, Li J
    Am J Pathol, 2014-01-30;184(4):944-52.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  46. CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFbeta / GARP-mediated mechanism.
    Authors: Miller M, Petty C, Tompkins M, Fogle J
    Virol J, 2014-01-18;11(0):7.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Neutralization
  47. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
    Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
    J Immunol, 2013-12-11;192(2):804-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  48. TGF-beta signaling regulates neuronal C1q expression and developmental synaptic refinement.
    Authors: Bialas A, Stevens B
    Nat Neurosci, 2013-10-27;16(12):1773-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunodepletion
  49. Cancer-associated fibroblasts might sustain the stemness of scirrhous gastric cancer cells via transforming growth factor-beta signaling.
    Authors: Hasegawa T, Yashiro M, Nishii T, Matsuoka J, Fuyuhiro Y, Morisaki T, Fukuoka T, Shimizu K, Shimizu T, Miwa A, Hirakawa K
    Int J Cancer, 2013-10-24;134(8):1785-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  50. Exosomes with membrane-associated TGF-beta1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction.
    Authors: Yu L, Yang F, Jiang L, Chen Y, Wang K, Xu F, Wei Y, Cao X, Wang J, Cai Z
    Eur J Immunol, 2013-06-21;43(9):2461-72.
    Species: Mouse
    Sample Types: Exosomes
    Applications: Neutralization
  51. Reduction of methylglyoxal-induced glycation by pyridoxamine improves adipose tissue microvascular lesions.
    Authors: Rodrigues T, Matafome P, Santos-Silva D, Sena C, Seica R
    J Diabetes Res, 2013-04-07;2013(0):690650.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  52. A dynamic dual role of IL-2 signaling in the two-step differentiation process of adaptive regulatory T cells.
    Authors: Guo Z, Khattar M, Schroder P, Miyahara Y, Wang G, He X, Chen W, Stepkowski S
    J Immunol, 2013-02-20;190(7):3153-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  53. Transforming growth factor beta induces sensory neuronal hyperexcitability, and contributes to pancreatic pain and hyperalgesia in rats with chronic pancreatitis.
    Authors: Zhu Y, Colak T, Shenoy M, Liu L, Mehta K, Pai R, Zou B, Xie X, Pasricha P
    Mol Pain, 2012-09-11;8(0):65.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  54. HIV-1 promotes intake of Leishmania parasites by enhancing phosphatidylserine-mediated, CD91/LRP-1-dependent phagocytosis in human macrophages.
    Authors: Lodge R, Ouellet M, Barat C, Andreani G, Kumar P, Tremblay MJ
    PLoS ONE, 2012-03-06;7(3):e32761.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  55. Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease.
    Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y
    J. Clin. Invest., 2011-10-17;121(11):4548-66.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  56. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011-07-09;54(10):2713-23.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  57. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition.
    Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ
    Mol. Biol. Cell, 2011-03-16;22(10):1686-98.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Neutralization
  58. Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity.
    Authors: Heise RL, Stober V, Cheluvaraju C, Hollingsworth JW, Garantziotis S
    J. Biol. Chem., 2011-03-11;286(20):17435-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  59. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
    Authors: Oida T, Weiner HL
    J. Immunol., 2010-08-18;185(6):3529-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  60. AIDS progression is associated with the emergence of IL-17-producing cells early after simian immunodeficiency virus infection.
    Authors: Campillo-Gimenez L, Cumont MC, Fay M, Kared H, Monceaux V, Diop O, Muller-Trutwin M, Hurtrel B, Levy Y, Zaunders J, Dy M, Leite-de-Moraes MC, Elbim C, Estaquier J
    J. Immunol., 2009-12-16;184(2):984-92.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  61. Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells.
    Authors: Pot C, Jin H, Awasthi A
    J. Immunol., 2009-07-01;183(2):797-801.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  62. Intrastromal keratotomy with femtosecond laser avoids profibrotic TGF-beta1 induction.
    Authors: Meltendorf C, Burbach GJ, Ohrloff C, Ghebremedhin E, Deller T
    Invest. Ophthalmol. Vis. Sci., 2009-04-22;50(8):3688-95.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  63. Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation.
    Authors: Hosui A, Kimura A, Yamaji D, Zhu BM, Na R, Hennighausen L
    J. Exp. Med., 2009-03-30;206(4):819-31.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ICC, Western Blot
  64. Vaccination route that induces transforming growth factor beta production fails to elicit protective immunity against Leishmania donovani infection.
    Authors: Bhowmick S, Mazumdar T, Ali N
    Infect. Immun., 2009-01-21;77(4):1514-23.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  65. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009-01-05;113(13):2955-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  66. Activation of Toll-like receptor 3 augments myofibroblast differentiation.
    Authors: Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M
    Am. J. Respir. Cell Mol. Biol., 2008-11-06;40(6):654-62.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Development, Neutralization
  67. Transforming growth factor-beta stimulates intestinal epithelial focal adhesion kinase synthesis via smad- and p38-dependent mechanisms.
    Authors: Walsh MF, Ampasala DR, Hatfield J, Vander Heide R, Suer S, Rishi AK, Basson MD
    Am. J. Pathol., 2008-06-26;173(2):385-99.
    Species: Human, Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  68. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
    Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
    Stem Cells, 2008-01-10;26(4):1047-55.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  69. Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection.
    Authors: Wan N, Dai H, Wang T, Moore Y, Zheng XX, Dai Z
    J. Immunol., 2008-01-01;180(1):113-21.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  70. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007-08-05;8(9):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  71. Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells.
    Authors: Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB
    J. Immunol., 2007-05-01;178(9):5552-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  72. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  73. IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells.
    Authors: Zheng SG, Wang J, Wang P, Gray JD, Horwitz DA
    J. Immunol., 2007-02-15;178(4):2018-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  74. Increased frequency of CD4+ cells expressing CD161 in cancer patients.
    Authors: Iliopoulou EG, Karamouzis MV, Missitzis I, Ardavanis A, Sotiriadou NN, Baxevanis CN, Rigatos G, Papamichail M, Perez SA
    Clin. Cancer Res., 2006-12-01;12(23):6901-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  75. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
    Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
    Blood, 2006-11-02;109(5):2234-42.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  76. Inhibition of p38 mitogen-activated protein kinase and transforming growth factor-beta1/Smad signaling pathways modulates the development of fibrosis in adriamycin-induced nephropathy.
    Authors: Li J, Campanale NV, Liang RJ, Deane JA, Bertram JF, Ricardo SD
    Am. J. Pathol., 2006-11-01;169(5):1527-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  77. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  78. Increase of CD4+ CD25+ regulatory T-cells in the liver of patients with hepatocellular carcinoma.
    Authors: Yang XH, Yamagiwa S, Ichida T, Matsuda Y, Sugahara S, Watanabe H, Sato Y, Abo T, Horwitz DA, Aoyagi Y
    J. Hepatol., 2006-03-09;45(2):254-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  79. Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression.
    Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY
    Immunol. Lett., 2006-03-03;105(2):159-66.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  80. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  81. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004-12-01;114(11):1640-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  82. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-09-16;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  83. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004-06-01;25(10):1652-9.
    Species: Human
    Sample Types: Serum
    Applications: Affinity Chromatography
  84. Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription.
    Authors: Jono H, Shuto T, Xu H, Kai H, Lim DJ, Gum JR, Kim YS, Yamaoka S, Feng XH, Li JD
    J. Biol. Chem., 2002-09-16;277(47):45547-57.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Neutralization
  85. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
    Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  86. Induction of a hypertrophic growth status of coronary smooth muscle cells is associated with an overexpression of TGF-beta.
    Authors: Schmidt A, 2019, Gopfert C, Vlodavsky I, Volker W, Buddecke E
    101211, 2002-03-01;81(3):138-44.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Neutralization
  87. Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
    Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
    J. Immunol., 2000-05-15;164(10):5337-43.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  88. Isoform specificity of commercially-available anti-TGF-beta antibodies.
    Authors: Mozes MM, Hodics T, Kopp JB
    J. Immunol. Methods, 1999-05-27;225(1):87-93.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot

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TGF-beta 1 Antibody
By Anonymous on 08/29/2019
Application: ELISA Sample Tested: Serum Species: Human
ELISA TGF-beta 1 Antibody MAB240
TGF‑ beta 1 Antibody
By Anonymous on 03/28/2019
Sample Tested: Recombinant protein Species: Mouse

I performed a competition ELISA to see if another company's TGFb1 had a similar epitope to two of R&D's antibodies . I coated my plate with 50ng/well of recombinant mouse TGF beta 1 protein (cat# 7666-MB) overnight. I washed and blocked with reagent diluent 2 DY995 for 2hr, washed and added another company's TGFb1 antibody at different concentrations 0.2-100ug/mL in reagent diluent 2 and incubated 1hr. I did not wash my wells but add the two antibodies MAB240-biotinylated it in-house and BAF at a final concentration of 2ug/mL and incubated 1hr diluted in reagent diluent 2. I washed and added streptavidin-HRP DY998 (1:200) and incubated 30 mins, washed and developed with the substrate pack DY999. I found MAB240 competed for binding to the mouse TGF beta 1 protein when the competitor's antibody was present but the chicken polyclonal was not inhibited.

TGF‑ beta 1 Antibody MAB240
TGF-beta 1 Antibody
By Anonymous on 08/22/2018
Application: IHC Sample Tested: A375 human melanoma cell line Species: Human
TGF-beta 1 Antibody
By CARLA RAMON on 08/08/2018
Application: IHC Sample Tested: Brain tissue Species: Mouse

10.1093/cercor/bhy164

Immunohistochemistry TGF-beta 1 Antibody MAB240
TGF-beta 1 Antibody
By Leslie Priddy on 04/01/2018
Application: ELISA Sample Tested: Tumor cell lyastes Species: Mouse
TGF-beta 1 Antibody
By Anonymous on 01/05/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the capture to build an ELISA for TGF-beta 1. BAF240 was used as the detection.

ELISA TGF-beta 1 Antibody MAB240
TGF-beta 1 Antibody
By Anonymous on 08/01/2017
Application: B/N Sample Tested: Blood monocytes Species: Human
TGF‑ beta 1 Antibody
By Anonymous on 04/26/2017
TGF-beta 1 Antibody
By Anonymous on 12/07/2016
Application: Flow Sample Tested: Breast cancer cells Species: Human

10^6 of MDA-MB-231 cells were stained for 30 minutes at 4C with 2.5 ug of Mouse TGF-β 1 antibody (red), or control antibody (black). Secondary: PE-Goat anti-Mouse IgG 1:1,000. Blocking: 1% BSA. Unfixed cells.

Flow Cytometry TGF-beta 1 Antibody MAB240