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Neurons – Structural Markers

Neurons are composed of distinct morphological regions with differing functions. These regions can be discriminated from each other by immunodetection of specific proteins that are localized to that specific region. This interactive graphic lists some of the most common markers used to identify the dendrites, axons, synapses, and growth cones of neurons. Clicking on a marker will take you to the R&D Systems selection of antibodies directed against that molecule. Clicking on a marker that is designated by (novusbio.com) will take you to the Novus Biologicals’ website and their antibody offerings for that protein.

Axon

Kinesin 5A

Kinesin 5B

L1CAM

beta-III Tubulin

NF-H

NF-L

Peripherin

Tau

Dendrite

ARC/ARG3.1

Drebrin 1

MAP2

SAP102

Growth Cone

BASP1

CAP1

CotL1

CRMP1

CRMP2

CRMP5

DCLK1

Doublecortin

Drebrin 1

FABP7/B-FABP

FARP2

GAP-43

GO Protein alpha

G protein alpha Inhibitor 2

Growth Cone Antibody

L1CAM

NCAM-1/CD56

Neuropilin-1

RTN1/NSP

SNAP25

SPG3A

STRAP

Syntaxin 7

Synapse

SHANK1

SHANK2

SHANK3

Bassoon

GAP-43

HOMER1

Neuroligin 1/NLGN1

Neuroligin 2/NLGN2

Neurexin 1 beta

PSD-95

Piccolo

SAP102

SNAP23

SNAP25

SV2A

Synapsin I

Synapsin II

Synaptogyrin 1

Gephyrin/GPHN

Synaptophysin

Synaptotagmin-1

Synaptotagmin-4

Syntaxin 1A

VAMP-1

VAMP-2

VGLUT1/SLC17A7

VIAAT/SLC32A1/VGAT

Neurexin 3

Fluorescent Synaptic
Vesicle Dyes

Fluorescent Synaptic
Activity Indicators

Data Examples

Immunocytochemistry-MAP2 in Human Embryonic Stem Cells

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MAP2 in Human Neurospheres. Microtubule-Associated Protein 2 (MAP2) was detected in immersion-fixed human embryonic stem cells/neurospheres using a Mouse Anti-Human MAP2 Monoclonal Antibody (R&D Systems, Catalog # MAB8304). Cells were stained using the NorthernLights^™ 557-Conjugated Donkey Anti-Mouse IgG Secondary Antibody (R&D Systems, Catalog # NL007; red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces.

Immunohistochemistry-Synaptotagmin-1 in Rat Spinal Cord

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Synaptotagmin-1 in Rat Spinal Cord. Synaptotagmin-1 was detected in perfusion-fixed frozen sections of rat spinal cord using a Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (R&D Systems, Catalog # MAB43641). The tissue was stained (red) and counterstained (green).

Immunohistochemistry-Paraffin: GAP-43 Antibody

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GAP-43 in Embryonic Mouse. GAP-43 was detected in immersion-fixed paraffin-embedded sections of embryonic mouse tissue (E15.5) using a Rabbit Anti-Human/Mouse/Rat Antigen Affinity-Purified Polyclonal Antibody (Novus Biologicals, Catalog # NB300-143). The tissue was stained with an Alexa Fluor^® 488-conjugated secondary antibody (green) and counterstained (blue).