Neurons - General Markers

In neural cell cultures and nervous tissue, neurons are identified by immunodetection of specific proteins localized to the surface of neurons and/or within the cell. The interactive graphic below lists some of the most commonly used neuronal markers including NeuN, Neuron-Specific Enolase (NSE), also called Enolase 2, Microtubule-Associated Protein 2 (MAP2), Neuron-Specific beta-III Tubulin, and PSD-95. Clicking on a molecule will take you to the R&D Systems product selection for researching that molecule. Clicking on a molecule that is designated by (novusbio.com) will take you to the Novus Biologicals’ website and their antibody offerings for that protein.

Calbindin D

Doublecortin

Enolase 2/Neuron-specific Enolase

MAP2

NeuN

NeuroD1

beta-III Tubulin

NF-H

NF-L

NF-M

PSD-93

PSD-95

UCH-L1/PGP9.5

Data Examples

View Larger

NeuN in Mouse Brain. NeuN, also called RBFOX3, was detected in immersion-fixed paraffin-embedded sections of mouse brain (hippocampus) using a Rabbit Anti-Human/Mouse/Rat RBFOX3/NeuN Antigen Affinity-Purified Polyclonal Antibody (Novus Biologicals, Catalog # NBP1-77686). The hippocampal tissue was stained using HRP and DAB (brown), and counterstained with hematoxylin (blue).

View Larger

beta-III Tubulin in Rat Cortical Neurons. beta-III Tubulin was detected in rat cortical neurons using a Mouse Anti-Neuron-Specific beta-III Tubulin Monoclonal Antibody (Clone # TuJ-1; R&D Systems, Catalog # MAB1195). The cells were stained using the NorthernLights^™ 557-Conjugated Donkey Anti-Mouse IgG Secondary Antibody (R&D Systems, Catalog # NL007; red). Rat astrocytes were also detected using a Sheep Anti-Human GFAP Antigen Affinity-Purified Polyclonal Antibody
(R&D Systems, Catalog # AF2594), followed by a NorthernLights^™ 493-Conjugated Donkey Anti-Sheep IgG Secondary Antibody (R&D Systems, Catalog # NL012; green).

View Larger

NF-H in Human Brain. Neurofilament (NF)-H was detected in immersion-fixed paraffin-embedded sections of human brain (cerebellum) using a Goat Anti-Human NF-H Antigen Affinity-Purified Polyclonal Antibody (R&D Systems, Catalog # AF3108). The tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems, Catalog # CTS008; brown) and counterstained with hematoxylin (blue).