For further assistance, please contact our technical service department.
Observation: High background level
Problem Corrective Action Insufficient washing Wash per protocol being sure to remove all Wash Buffer from wells before addition of next component Contamination with alkaline phosphatase Keep work area clean and free of alkaline phosphatase
Observation: Poor precision
Problem Corrective Action Plate not washed before use Wash per protocol RNase contamination Use RNase-free technique Pipetting error Use a new pipet tip for each pipetting step and use proper technique
Observation: No signal for positive control
Problem Corrective Action Component or step omitted Read protocol thoroughly before repeating the assay RNase contamination Use RNase-free technique