iPSCs and 3-D cell culture model systems such as organoids are being increasingly used as in vitro model systems for studying human organ development and disease, screening for drug efficacy or toxicity, and investigating personalized medicines.
Take your research to the next level with our wide selection of Cultrex® basement membrane extracts (BME) and R&D Systems™ matrix-regulating proteins and growth factors designed to promote robust, reproducible organoid culture conditions. Browse our selection today and let Bio-Techne help advance your 3-D cell culture research.
Limited time bundle promotion:
- Choose your matrix of interest
- Choose a recombinant protein, either a growth factor or a matrix-regulating protein, or both
- Place your order using promo code PROTBME15 and receive 15% off your order
Browse Products Below & Order Online Today
For questions, please contact cgt.cellculture@bio-techne.com in North America or sales.emea@bio-techne.com in Europe.
Cultrex Basement Membrane Extracts Product Table
| Product Name | Size | Catalog # |
| Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract | 5 mL | BME001-05 |
| 10 mL | BME001-10 | |
| Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear | 5 mL | 3533-005-02 |
| 10 mL | 3533-010-02 | |
| Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear | 5 mL | 3433-005-01 |
| 10 mL | 3433-010-01 | |
| Cultrex Reduced Growth Factor Basement Membrane Extract, Type R1 | 5 mL | 3433-005-R1 |
| 10 mL | 3433-010-R1 |
Matrix-Regulating Proteins Product Table
| Protein (Human) | Source | Pack Size | Catalog # |
| Decorin | Sf 21 | 100 ug | 143-DE |
| Nectin-4 | NS0 | 50 ug | 2659-N4 |
| Nectin-4 | HEK293 | 50 ug | 10682-N4 |
| Nidogen-1/Entactin | NS0 | 50 ug | 2570-ND |
| Pro-Collagen I alpha 1/COL1A1 | CHO | 20 ug | 6220-CL |
Growth Factors Product Table
| Protein (Human) | Source | Pack Sizes | Catalog # |
| BMP-2 | CHO | 10, 50, 100 ug | 355-BM* |
| FGF-10 | E. coli | 25, 250 ug | 345-FG* |
| Noggin | NS0 | 25, 100, 1000 ug | 6057-NG* |
| Noggin | NS0 | 50, 100, 500 ug | 3344-NG |
| TGF-beta 1 | HEK293 | 5, 25, 100 ug | 7754-BH* |
*Carrier and Carrier Free versions of these proteins are available.
Terms and conditions: Offer is valid until September 30, 2023. Receive 15% off your order when you purchase a select BME from our catalog, along with a minimum of at least one select growth factor or matrix-regulating protein. Your order must contain at least one BME and one recombinant protein in order to qualify. BMEs included in the offer: BME001-05, BME-001-10, 3533-005-02, 3533-010-02, 3433-005-01, 3433-010-01, 3433-005-R1, 3433-010-R1. Proteins included in the offer: 143-DE, 2659-N4, 10682-N4, 2570-ND, 6220-CL, 355-BM, 345-FG, 6057-NG, 3344-NG, 7754-BH. 1 ml sizes of BME are excluded from this offer. Promo code PROTBME15 must be quoted at time of the order. Offer is valid for customers in North America and Europe only. Promotion not limited to one use per customer. Offer only valid on orders placed directly with Bio-Techne and excludes Bio-Techne distributors. Offer excludes bulk quantities, custom, and special orders. Other exclusions may apply. Offer cannot be used in conjunction with any other offers or discounts, for prior purchases, or orders pending. No credits will be issued. All requests subject to availability and approval by Bio-Techne.
R&D Systems Proteins for Organoid Culture are Frequently More Active than Leading Competitor’s Proteins and Display High Lot-to-Lot Consistency
R&D Systems Recombinant Human Noggin is 30-fold More Active than Recombinant Human Noggin from a Leading Competitor and Displays Minimal Lot-to-Lot Variability. (A) Using the ATDC5 mouse chondrogenic cell line, the ability of R&D Systems Recombinant Human Noggin (Catalog # 6057-NG; orange line) or a top competitor’s recombinant human noggin (green line) to inhibit alkaline phosphatase production stimulated by Recombinant Human BMP-4 (R&D Systems, Catalog # 314-BP; 50 ng/mL) was assessed. The ED50 for R&D Systems Recombinant Human Noggin using this assay was approximately 30-fold higher than Noggin obtained from a leading competitor. (B) Using the same assay described in part (A), the lot-to-lot consistency of R&D Systems Recombinant Human Noggin (Catalog # 6057-NG) was assessed by side-by-side testing of the bioactivity of three independent lots of the protein (purple, light blue, orange trace on the graph). The dark blue trace on the graph is showing the ability of Recombinant Human BMP-4 (R&D Systems, Catalog # 314-BP; 50 ng/mL) to induce alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. (C, D) BG01V human embryonic stem cells were cultured in Mouse Embryonic Fibroblast Conditioned Media supplemented with FGF basic (5 ng/mL). Stem cells were driven into early cells of the neuroectoderm using a 3-day incubation in Recombinant Human Noggin (25 ug/mL) from either R&D Systems (Lot 1, Lot 2; Catalog # 6057-NG) or from two different competitors (Competitor 1, Competitor 2). Control cells were incubated in media without Noggin (No Noggin). The cells were stained for the early ectoderm marker, Otx2, and the neuroectoderm marker, SOX1. (C) Representative images of Otx2 (red), SOX1 (green), and DAPI (blue) staining in embryonic stem cells differentiated with Noggin from R&D Systems or Noggin from Competitor 2. (D) SOX1+ clusters were quantified under each of the indicated culture conditions. Cells treated with R&D Systems Noggin showed an increase in SOX1+ cells compared to both untreated and competitor-treated cells. R&D Systems Noggin showed consistent differentiation across the lots tested. BG01V human embryonic stem cells were licensed from ViaCyte, Inc.
Human Lung Organoids Embedded in Cultrex UltiMatrix RGF Basement Membrane Extract and Cultured Using R&D Systems Proteins. Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (Catalog # BME001-05) and cultured for 20-60 days in lung organoid expansion medium, which includes Recombinant Human FGF-10 (Catalog # 345-FG) and Recombinant Human Noggin(Catalog # 6057-NG), along with the other reagents listed in the lung organoid expansion medium recipe in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).
Customer Success Story Using Our Organoid-Related Workflow Solutions
“Our research is greatly facilitated by Bio-Techne products. The various organoid systems and co-cultures are all performed in or on R&D Systems Cultrex Reduced Growth Factor BME with great results. Even for more exotic organoids, such as snake venom gland organoids, we could achieve breakthroughs with R&D Systems growth factors and BME as well as spatial visualization of toxin transcripts using ACD’s RNAScope.”
Jens Puschhof | Hans Clevers Lab | Hubrecht Institute, The Netherlands
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