Human beta-Arrestin 1 Antibody

Name: Human beta-Arrestin 1 Antibody
Brand: R&D Systems
SKU: MAB45601
Rating: 5 (1 reviews)

Catalog #: MAB45601 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB45601
MAB45601-SP

Detection of Human beta ‑Arrestin 1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta-Arrestin 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human beta -Arrestin 1 in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 425817

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human beta -Arrestin 1
Met1-Arg418
Accession # P49407

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human colon cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human beta ‑Arrestin 1 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Rat Anti-Human beta -Arrestin 1 Monoclonal Antibody (Catalog # MAB45601) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for beta -Arrestin 1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Arrestin 1

The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 ( beta -Arrestin 1), Arrestin 3 ( beta -Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, beta -Arrestins 1 and 2 are ubiquitously expressed, and were initially described as negative regulators of G protein-coupled receptor (GPCR) signaling. More recently, beta -Arrestins have been determined to serve as scaffolds for various signaling pathways, including the MAPK cascades activating ERK2, p38 alpha, and JNK3. These beta -Arrestin scaffolds tie together the appropriate kinases in series, forming a discreet signaling module that localizes components to specific subcellular environments and facilitates greater kinase activation.

Entrez Gene IDs

408 (Human); 109689 (Mouse); 25387 (Rat)

Alternate Names

ARB1; ARR1; ARRB1; arrestin 2; Arrestin beta-1; arrestin, beta 1; betaArrestin 1; beta-Arrestin 1; beta-arrestin-1