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Human BMPR-II Antibody

Catalog #: AF811
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF811
AF811-SP
BMPR-II in Human Prostate.
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Product Details
Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMPR-II Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMPR-II in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMPR-II
Ala26-Ile151
Accession # Q13873
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry BMPR-II antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

BMPR-II in Human Prostate. BMPR-II was detected in formalin fixed paraffin-embedded sections of human prostate using Goat Anti-Human BMPR-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow Cytometry Detection of BMPR‑II antibody in PC‑3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of BMPR‑II in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Goat Anti-Human BMPR-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMPR-II

Like other members of the TGF-beta superfamily, cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the binding to heteromeric complex of type I and type II serine-threonine kinase receptors. Both receptor types are required for the signal transduction. BMP receptor II (BMPR-II) is one of the five mammalian type II receptors (including TGF-beta R-II, ActR-II, ActR-IIB, BMPR-II, and MISR-II) for the TGF-beta superfamily ligands. The type II receptors for TGF-beta and activin bind ligands with high affinity by itself. In contrast, BMPR-II binds BMP-2, BMP-4, and BMP-7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor. BMPR-II mRNA is widely expressed in fetal and adult tissues. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity (1-3).

References
  1. Rosenzweig, B.L. et al. (1995) Proc. Natl. Acad. Sci. USA 92:7632.
  2. Beppu, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:499.
  3. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
Long Name
Bone Morphogenetic Protein Receptor II
Entrez Gene IDs
659 (Human); 12168 (Mouse)
Alternate Names
BMP type II receptor; BMP type-2 receptor; BMPR2; BMPR-2; BMPR3; BMPRII; BMPR-II; BMPR-IIprimary pulmonary hypertension 1; Bone morphogenetic protein receptor type II; bone morphogenetic protein receptor type-2; bone morphogenetic protein receptor, type II (serine/threonine kinase); BRK-3; EC 2.7.11; EC 2.7.11.30; FLJ41585; FLJ76945; PPH1BMR2; T-ALK; type II activin receptor-like kinase; type II receptor for bone morphogenetic protein-4

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Citations for Human BMPR-II Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Age-related expression of TGF beta family receptors in human cumulus oophorus cells
    Authors: A Ribeiro, C Freitas, L Matos, A Gouveia, F Gomes, JL Silva Carv, H Almeida
    J. Assist. Reprod. Genet., 2017-05-02;0(0):.
  2. Endocytosis contributes to BMP2-induced Smad signalling and neuronal growth
    Authors: SV Hegarty, AM Sullivan, GW O'Keeffe
    Neurosci. Lett, 2017-02-08;643(0):32-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Epigenetic repression of bone morphogenetic protein receptor II expression in scleroderma.
    Authors: Wang Y, Kahaleh B
    J Cell Mol Med, 2013-07-16;17(10):1291-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. BMP4 is a peripherally-derived factor for motor neurons and attenuates glutamate-induced excitotoxicity in vitro.
    Authors: Chou H, Lai D, Huang C, McLennan I, Wang H, Wang P
    PLoS ONE, 2013-03-05;8(3):e58441.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro.
    Authors: Fong D, Bisson M, Laberge G, McManus S, Grenier G, Faucheux N, Roux S
    Cell Signal, 2013-01-08;25(4):717-28.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  6. Role of BMPR2 Alternative Splicing in Heritable Pulmonary Arterial Hypertension Penetrance
    Authors: Joy Cogan, Eric Austin, Lora Hedges, Bethany Womack, James West, James Loyd et al.
    Circulation
  7. Abcc6 deficiency causes increased infarct size and apoptosis in a mouse cardiac ischemia-reperfusion (I/R) model
    Authors: Imran N. Mungrue, Peng Zhao, Yucheng Yao, Haijin Meng, Christoph Rau, Jocelyn V. Havel et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  8. Bone morphogenetic protein receptor IB as a marker for enrichment of osteogenic precursor-like cells in human dermis.
    Authors: He J, Dong J, Wang T
    Arch. Dermatol. Res., 2011-06-05;303(8):581-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Transcripts from a novel BMPR2 termination mutation escape nonsense mediated decay by downstream translation re-initiation: implications for treating pulmonary hypertension
    Authors: R Hamid, L K Hedges, E Austin, J A Phillips, J E Loyd, J D Cogan
    Clinical Genetics
  10. High-density lipoproteins affect endothelial BMP-signaling by modulating expression of the activin-like kinase receptor 1 and 2.
    Authors: Yao Y, Shao ES, Jumabay M, Shahbazian A, Ji S, Bostrom KI
    Arterioscler. Thromb. Vasc. Biol., 2008-10-23;28(12):2266-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  11. Cell origin in experimental repair of cricoid cartilage defects treated with recombinant human bone morphogenetic protein-2.
    Authors: Tcacencu I, Carlsoo B, Stierna P
    Wound Repair Regen, 2005-05-01;13(3):341-9.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  12. The combined mechanism of bone morphogenetic protein- and calcium phosphate-induced skeletal tissue formation by human periosteum derived cells.
    Authors: Bolander J, Ji W, Geris L et al.
    Eur Cell Mater.

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