Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca fuscata (Japanese Macaque)

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FABP7/B-FABP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FABP7/B-FABP
Met1-Ala132
Accession # O15540

Specificity

Detects human FABP7/B-FABP in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP6, rhFABP8, rhFABP9 and recombinant mouse FABP9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FABP7/B‑FABP Antibody

FABP7 antibody in Human Melanoma by Immunohistochemistry (IHC-P).

FABP7 in Human Melanoma.

FABP7 was detected in immersion fixed paraffin-embedded sections of human melanoma using 5 µg/mL Goat Anti-Human FABP7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human FABP7/B-FABP antibody by Simple WesternTM.

Detection of Human FABP7/B‑FABP by Simple WesternTM.

Simple Western lane view shows lysates of human cerebellum tissue and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP7/B-FABP at approximately 18 kDa (as indicated) using 50 µg/mL of Goat Anti-Human FABP7/B-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human FABP7/B-FABP by Western Blot

Detection of Human FABP7/B-FABP by Western Blot

FABP7 expression in RCC cell lines. A) Q-PCR analysis was performed using total RNA extracted from a human embryonic kidney (HEK293) and RCC (TUHR14TKB, OS-RC-2, 786-O, 769-P, Caki-1, and ACHN) cell lines. FABP7 expression was determined with Q-PCR by the primers listed in Table 2. B) HEK293 and RCC (TUHR14TKB, OS-RC-2, 786-O, 769-P, Caki-1, and ACHN) cells were cotransfected with the -1122+89 pGL4-FABP7 promoter construct, and cultured for one day. Extracts prepared from transfected cells were assayed for luciferase activity. FABP7 promoter activities were normalized to the control's Renilla luciferase activity. The results shown are an average of four independent experiments with standard deviations indicated by the error bars. C) Western blot analysis was performed using cytoplasmic extracts from HEK293 and RCC (TUHR14TKB, OS-RC-2, 786-O, 769-P, Caki-1, and ACHN) cell lines. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21771320), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human FABP7/B‑FABP Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma and astrocytoma

Simple Western

50 µg/mL
Sample: Human cerebellum tissue and human hippocampus tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human FABP7

Reviewed Applications

Read 2 reviews rated 4 using AF3166 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP7/B-FABP

FABP7, also known as (Brain) B-FABP, Brain lipid-binding protein (BLBP), and mammary-derived growth inhibitor-related (MRG), belongs to the family of intracellular lipid-binding proteins. It is involved in fatty acid uptake and intracellular transport and is important in brain development. Human and mouse FABP7 share 86% amino acid sequence identity.

Long Name

Fatty Acid-Binding Protein 7

Alternate Names

B-FABP, BFABP, BLBP

Entrez Gene IDs

2173 (Human); 12140 (Mouse); 80841 (Rat)

Gene Symbol

FABP7

UniProt

O15540

Additional FABP7/B-FABP Products

Product Documents for Human FABP7/B‑FABP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FABP7/B‑FABP Antibody

For research use only

Citations for Human FABP7/B‑FABP Antibody

Customer Reviews for Human FABP7/B‑FABP Antibody (2)

4 out of 5
2 Customer Ratings
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21771320
    Species: Rat
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 22646866
    Species: Human
    Verified Customer | Posted 01/07/2015

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Protocols

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