Human/Mouse MMP-24/MT5-MMP Antibody

Catalog #: MAB924 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB924
MAB924-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse MMP-24/MT5-MMP Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse MMP‑24/MT5-MMP in direct ELISAs and detects human and mouse MMP‑24/MT5-MMP in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-9, and the catalytic domains of recombinant human MMP‑14, 15, and 16 is observed.

Source

Monoclonal Rat IgG_2A Clone # 143908

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse MMP‑24/MT5-MMP
Arg125-Gln296
Accession # Q9R0S2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human/Mouse MMP‑24/MT5-MMP

Flow Cytometry

0.25 µg/10⁶ cells

A172 human glioblastoma cell line and bEnd.3 mouse endothelioma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-24/MT5-MMP

MMP-24, also known as MT5-MMP, is a 62-65 kDa member of the M10 metallopeptidase family, a subfamily of MMPs. It is a type I transmembrane glycoprotein that is converted into a 58 kDa active form (amino acids [aa] 129-618) following removal of its propeptide. Proteolytic cleavage after Arg554 generates a 52 kDa active, soluble form that undergoes further cleavage in to 27-34 kDa fragment. MMP-24 has restricted expression, being found on neurons, mast cells, neural stem cells and ependymal cells lining the ventricles of the brain. It is known to cleave chondroitin sulfate, dermatan sulfate, pro-MMP-2, and N-Cadherin. Its activation of MMP-2 likely contributes to neurite outgrowth, while its cleavage of N-Cadherin in-trans between neural stem cells and ependymal initiates stem cell expansion and proliferation. Over amino acids 125-296, mouse MMP-24 shares 100% and 99% aa sequence identity with rat and human MMP-24, respectively.

Long Name

Matrix Metalloproteinase 24/Membrane Type 5 MMP

Entrez Gene IDs

10893 (Human); 17391 (Mouse)

Alternate Names

EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 24 (membrane-inserted); matrix metalloproteinase 24 (membrane-inserted); matrix metalloproteinase-24; membrane-type 5 matrix metalloproteinase; Membrane-type matrix metalloproteinase 5; Membrane-type-5 matrix metalloproteinase; MMP24; MMP-24; MT5MMP; MT5-MMP; MT5-MMPMMP25; MT-MMP 5; MTMMP5; MT-MMP5