Human/Mouse/Rat ERK4 Antibody

Catalog #: MAB3914 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3914
MAB3914-SP

Detection of Human and Mouse ERK4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ERK4 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat ERK4.

Source

Monoclonal Rat IgG_2A Clone # 355810

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ERK4
Met1-Ala126
Accession # P31152

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse ERK4 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for ERK4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry

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ERK4 in Raji Human Cell Line. ERK4 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK4

Extracellular signal-regulated kinase 4 (ERK4) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 4 (MAPK4), p63MAPK, and ERK3‑related kinase. ERK4 and the structurally similar ERK3 (MAPK6) lack the phosphoacceptor sequence T-X-Y present in other MAPKs. ERK4 complexes with ERK3 to activate MAPK-activated protein kinase 5 (MK5), also known as PRAK.

Long Name

Extracellular Signal-regulated Kinase A

Entrez Gene IDs

5596 (Human); 225724 (Mouse); 54268 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.24; ERK3; Erk3-related; ERK4; ERK-4; Extracellular signal-regulated kinase 4; MAP kinase 4; MAP kinase isoform p63; MAPK 4; MAPK4; mitogen-activated protein kinase 4; p63Mapk; PRKM4; PRKM4p63-MAPK