Human/Mouse/Rat PKLR Antibody

Catalog #: AF8519 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8519
AF8519-SP

Detection of Human, Mouse, and Rat PKLR by Western Blot.

2 Images

All PKLR Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PKLR Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat PKLR in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human PKM-2 is observed.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PKLR
Leu47-Ser574
Accession # P30613

Formulation

Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Simple Western

0.5 µg/mL

Human Liver and Mouse Liver

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat PKLR by Western Blot. Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat PKLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8519) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKLR at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human and Mouse PKLR by Simple Western^TM. Simple Western lane view shows lysates of human liver and mouse liver, loaded at 0.2 mg/mL. A specific band was detected for PKLR at approximately 57 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat PKLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8519). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: PKLR

Human PKLR (Pyruvate kinase PKLR) is a 60 kDa, cytosolic red cell/liver pyruvate kinase that belongs to the pyruvate kinase family of proteins. It is 574 amino acids (aa) in length, and contains two Allosteric activator binding regions. PKLR is allosterically activated by fructose 1,6-bisphosphate. Pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. Additional shorter isoform apparently exists. It is 543 aa in length and shows a two aa substitution for aa's 1-33. Human PKLR is 93% aa identical to mouse and rat PKLR.

Long Name

Pyruvate Kinase, Liver And RBC

Entrez Gene IDs

5313 (Human); 18770 (Mouse)

Alternate Names

EC 2.7.1.40; PK1; PK1PKRL; PKL; PKLR; PKR; Pyruvate Kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase isozymes R/L; pyruvate kinase type L; pyruvate kinase, liver and blood cell; pyruvate kinase, liver and RBC; Red cell/liver pyruvate kinase; RPK; R-type/L-type pyruvate kinase

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.