Human Phospho-GSK-3 beta (S9) Antibody

Catalog #: MAB8934 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8934
MAB8934-SP

Detection of Human Phospho-GSK‑3 beta (S9) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-GSK-3 beta (S9) Antibody Summary

Species Reactivity

Human

Specificity

Detects human GSK‑3 beta when phosphorylated at S9 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1253C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human GSK‑3 beta S9 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Immunocytochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-GSK‑3 beta (S9) by Western Blot. Western blot shows lysates of untreated (-) or MCF-7 human breast cancer cell line treated (+) with 100 ng/ml Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes and HeLa human cervical epithelial carcinoma cell line treated (+) with 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-GSK-3 beta Monoclonal Antibody (Catalog # MAB8934) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-GSK-3 beta (S9) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Phospho-GSK-3 beta (S9) in MCF‑7 Human Cell Line. GSK-3 beta phosphorylated at S9 was detected in immersion fixed MCF-7 human breast cancer cell line untreated (lower panel) or treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1; upper panel) using Rabbit Anti-Human Phospho-GSK-3 beta Monoclonal Antibody (Catalog # MAB8934) at a 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

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Detection of Human Phospho-GSK‑3 beta (S9) by Simple Western^TM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-GSK-3 beta (S9) at approximately 56 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-GSK-3 beta Monoclonal Antibody (Catalog # MAB8934). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified asan inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Long Name

Glycogen Synthase Kinase 3 beta

Entrez Gene IDs

2932 (Human); 56637 (Mouse); 84027 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform